体外诱导人骨髓间充质干细胞定向肝细胞分化  

In vitro differentiation of human bone marrow derived mesenchymal stem cells into heoatocytes

作　　者：梁峰[1] 王韫芳[1] 岳慧敏[1] 南雪[1] 施双双[1] 闫舫[1] 李荣[2] 裴雪涛[1] 

机构地区：[1]军事医学科学院输血医学研究所干细胞与再生医学研究室,北京100850 [2]解放军总医院普通外科研究所,北京100853

出　　处：《军医进修学院学报》2006年第3期230-232,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家重点基础研究发展规划项目(973项目;2001CB509906;2005CB522702);国家自然科学基金项目(30400415)

摘　　要：目的:研究人骨髓间充质干细胞向肝细胞的诱导分化,为肝组织工程提供理想的细胞来源。方法:以肝细胞生长因子、成纤维细胞生长因子4对种植于基底膜基质中的人骨髓间充质干细胞进行三维立体诱导培养,在培养后不同时间,分别进行形态观察、AFP、Alb的免疫组化荧光染色及靛青绿的摄取与排泌试验。结果:该条件下诱导后的人骨髓间充质干细胞生成肝细胞样细胞,阳性表达肝细胞特有表面标志AFP、Alb,并具备肝细胞特有的摄取与排泌靛青绿的活性功能。结论:肝细胞生长因子、成纤维细胞生长因子4及基底膜基质可能在体外人骨髓间充质干细胞向肝细胞分化的过程中提供适宜的微环境并起重要作用,这为间充质干细胞在肝组织工程中的应用提供了理论与技术上的支持。Objective：To study differentiation of human bone marrow derived mesenchymal stem cells （BDMSC） into heoatecytes for ideal cell origin of liver tissue engineering. Methods：After vary days of induction with hepatecyte growth factor （HGF） and fibroblast growth factor 4 （FGF-4） in 3D Matrigel, BDMSC and heoatocyte-like cells were determined by the utilization of morphological observation, immunofluorescence of alpha-fetoprotein （AFP） and albumin （Alb）and uptake and excretion of Indecyanine green（ICG） that were special for heoatecytes. Results：After 3D-cultured and induced with HGF and FGF-4 in vitro in Matrigel for 3-7 days, BDMSCs came into heoatecyte-like cells that expressed AFP and Alb and possessed ability of uptake and excretion of ICG. Conclusion：In vitro HGF,FGF and Matrigel maybe supply a proper microenviroment and play an important role during process of heoatecytes differentiation of BDMSC. This study offered a theoretical and technical foundation of mesenchymal stem cells （MSC） application for liver tissue engineering.

关 键 词：骨髓间充质干细胞 肝细胞 肝细胞生长因子 碱性成纤维细胞生长因子2 

分 类 号：Q813[生物学—生物工程]