涎腺腺样囊性癌p16-pRB-cyclinD1通路的研究  

Study on the RB Pathway p16-pRB-cyclinD1 in adenoid cystic carcinoma of salivary glands

作　　者：陈玲[1] 万飞[1] 

机构地区：[1]南京医科大学附属江苏省口腔医院病理科,江苏省南京市210029

出　　处：《实用老年医学》2006年第3期166-168,F0004,共4页Practical Geriatrics

基　　金：南京医科大学附属江苏省口腔医院人才引进科研启动基金(KY2003001)

摘　　要：目的探讨视网膜母细胞瘤蛋白(pRB)、p16蛋白、细胞周期蛋白D1(cyclinD1)在涎腺腺样囊性癌(ACC)中的表达及相互关系。方法收集5个正常的唾液腺标本,27例≥60岁ACC患者标本,应用S-P免疫组化方法观察RB通路中pRB、p16蛋白和cyclinD1的表达,增殖细胞核抗原(PCNA)用于细胞增殖的评价。结果pRB在正常唾液腺和ACC中表达是一致的。在4例(4/27,14·8%)观察到p16蛋白无表达。6例(22·2%,6/27)病例中观察到cyclinD1的过度表达,其中4例p16蛋白无表达,以实性ACC为主。结论涎腺ACC中p16蛋白缺失,cyclinD1的过度表达与ACC的肿瘤细胞高度增殖、肿瘤高恶性度及病人预后有关。Objective To explore the expression of pRB, p16, cyclinDl protein and its significance to adenoid cystic carcinoma of salivary glands（ACC）. Methods Immunohistochemical staining was performed in 5 specimens of normal salivary glands and 27 cases（≥60 years old） of ACC. The RB Pathway（ pRB-p16INK4A-cyclinD1 ） in ACC was observed. Proliferating cell nuclear antigen（PCNA） labelling index was used for the evaluation of cell proliferation. Results The level of expression of pRB was found to be the same both in nomal salivary glands and ACC. p16 was negative in 4 of 27 cases of ACC. p16 was negative in 4 of the 6 cases of ACC which over-expressed cyclinD1, and the 4 cases of ACC showed solid type. Conclusions p16 is absent in ACC, and the over expression of cyclinDl correlates to the cell proliferation of ACC, malignancy of tumor and poor prognosis.

关 键 词：涎腺肿瘤 视网膜母细胞瘤蛋白质 免疫组织化学 老年人 

分 类 号：R392.3[医药卫生—免疫学] R793.8[医药卫生—基础医学]