低剂量中子辐射对大鼠肾组织细胞外基质成分表达的影响  

Effects of Neutron Radiation On Expression of Extracellular Matrix in Renal Tissue

作　　者：彭武林[1] 陈铁河[1] 蒋定文[1] 鲁永杰[1] 杨翊芳[1] 张爱兵[1] 

机构地区：[1]海军医学研究所,上海200433

出　　处：《中国辐射卫生》2006年第2期138-139,共2页Chinese Journal of Radiological Health

摘　　要：目的研究低剂量中子辐射对大鼠肾组织细胞表达细胞外基质成分的影响。方法大鼠随机分4组,分别接受不同剂量的中子照射后,取肾组织用胶原酶消化以分散细胞,采用放射免疫方法测定原代培养细胞上清中的细胞外基质成分的含量。结果0.29Gy和1.21Gy的中子对HA和PCⅢ的影响不明显,但都显著刺激LN的增加;0.62Gy的中子可显著抑制HA和PCⅢ,对LN的影响却不明显。中子对三种基质成分影响的量效规律有着一致性,均表现为中剂量(0.62Gy)组的测定值比低剂量组和高剂量组的测定值低。结论低剂量中子辐射可引起大鼠肾组织细胞分泌细胞外基质成分发生变化。Objective To determine the effcet of neutron radiation on expression of extracellular matrix（ECM）in renal tissue. Methods Renal cells separated from rats were exposed at 0.26-1.21 Gy neutron, and were maintained in serum-free medium for a further 48 h. Primary cultures were tested with RIA methods for expression of hyaluronan（HA）, laminin（LN） and preeollagenⅢ （PC Ⅲ ）. Results HA and PC Ⅲwere redueed remarkably by 0.62 Gy neutron, and were not effeeted by 0.29 Gy and 1.21 Gy neutron; Irradiated with dose of 0.29 Gy and 1.21 Gy neutron could lead to a remarkable elevation of Ⅲ, but 0.62 Gy neutron has no effects to LN. Conclusion Lower dosage of neutron could lead to an altered production of ECM in rat renal tissue.

关 键 词：中子 细胞外基质 透明质酸 层粘连蛋白 Ⅲ型前胶原 

分 类 号：Q691.5[生物学—生物物理学]