菠萝17份种质的ISSR分析  

ISSR Analysis on 17 Pine Apple Resources

作　　者：张如莲[1] 傅小霞[1] 漆智平[1] 陈业渊[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,海南儋州571737

出　　处：《中国农学通报》2006年第6期428-431,共4页Chinese Agricultural Science Bulletin

基　　金：科技部科技基础性工作和社会公益研究专项"热带作物种质资源DNA指纹图谱鉴定技术标准研究"(2003DIB7J066)的部分内容。

摘　　要：利用ISSR分子标记技术对17份菠萝种质进行了基因组DNA多态性分析。从62个ISSR引物中筛选出6个多态性引物用于PCR扩增,共扩增出70条DNA条带,其中多态性条带44条,所占比例为62.86%,平均每个引物扩增的DNA条带的数目为11.67条。6个品种具有特异性的扩增带,可作为种质鉴定的依据。根据ISSR扩增结果建立的UPGMA聚类图,在距离系数约0.1处可把供试的17份菠萝种质分为3大类。The polymorphism of 17 Pine apple cultivars were analyzed by inter-simple sequence repeat（ISSR）.6 primers were selected from 62 ISSR primers, and a total of 70 DNA bands were amplified, 44 of which（62.86%）were polymorphic. The average number of DNA bands amplified by each primer was 11.67. There were specific amplified bands in six cultivars, and these bands can be used in Pine apple identification. The dendrogram constructed by UPGMA showed that the 17 individuals were classified into three groups. Genetic relationships of Pine apple were also discussed.

关 键 词：菠萝 ISSR 品种鉴定 遗传关系 

分 类 号：S667.9[农业科学—果树学]