条斑紫菜粗多糖对淋巴细胞和支持细胞的增殖作用  

Effects of Crude Polysaccharide from Porphyra yezoensis on the Lymphocytes and the Sertoli Cells

作　　者：郭婷婷[1] 张陆曦[1] 顾佳雯[1] 刘凤[1] 何培民[1] 

机构地区：[1]上海水产大学水产种质资源发掘与利用省部级重点实验室,上海200433

出　　处：《生物技术通讯》2006年第3期359-361,共3页Letters in Biotechnology

基　　金：上海科委重点科技攻关计划项目(054319936);上海教委优势(重点)学科资助项目(Y1101)

摘　　要：目的:探讨条斑紫菜粗多糖的一些生物学功能,包括促进淋巴细胞增殖以及促进支持细胞生长的功能。方法:将原代培养的大鼠和小鼠脾淋巴细胞以及大鼠睾丸支持细胞分别接种于96孔板中,将不同浓度的条斑紫菜粗多糖(0.05、0.5、5、10mg/mL)加入培养,每种浓度设6个复孔,并设对照组孔。采用四氮甲唑蓝(MTT)法检测细胞的增殖率。结果:条斑紫菜粗多糖能够显著地促进大鼠、小鼠脾淋巴细胞和大鼠睾丸支持细胞的增殖,增殖率随多糖浓度的提高而升高;当多糖浓度为10mg/mL时,增殖率分别为137.3%、149%和454.5%。结论:条斑紫菜粗多糖具有提高免疫力及促进生殖功能的作用,其对睾丸支持细胞的增殖作用目前还未见报道。Objective： In order to research some biological functions of crude polysaccharide from Porphyra yezoensis, the lymphocytes and the sertoli cells were deteced. Methods： After primary culturing, the spleen lymphocytes of mice and rats and the sertoli cells were separated into 96-well cell culture plates. Different concentrations of crude polysaccharide from P.yezoensis（0.05, 0.5, 5, 10 mg/mL） were selected and added to the culture. The cell survival rates were assessed by MTT assay. Results： Obviously, the crude polysaccharide from P.yezoensis can enhance the proliferation of these cells. When the concentration of the polysaccharide is 10 mg/mL, the rate of the proliferation reached 137.3%, 149% and 454.5% respectively. Conclusion： The results indicates that crude polysaccharide from P.yezoensis may improve the immu- nity and the procreative function. Especially, the effect on the proliferation of the sertoli cells has not been reported yet.

关 键 词：条斑紫菜粗多糖 脾淋巴细胞 睾丸支持细胞 MTT法 原代培养 

分 类 号：Q492.4[生物学—生理学] TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程]