宁夏及其周边地区博尔纳病病毒感染的分子流行病学研究  

Study on molecular epidemiology of Borna disease virus in Ningxia and vicinal regions

作　　者：王振海[1] 谢鹏[2] 韩玉霞[3] 詹军[3] 

机构地区：[1]宁夏医学院附属医院综合病房,银川750004 [2]重庆医科大学附属第一医院神经内科重庆市神经病学重点实验室 [3]宁夏回族自治区疾病预防控制中心

出　　处：《中华流行病学杂志》2006年第6期479-482,共4页Chinese Journal of Epidemiology

基　　金：国家自然科学基金资助项目(30470605);宁夏卫生厅基金重点资助项目(W2004021)

摘　　要：目的探讨博尔纳病病毒(BDV)在宁夏及其周边地区的流行状况。方法采用巢式逆转录酶聚合酶链反应结合荧光定量PCR检测了52例病毒性脑炎(VE)患者和32名健康人外周血液(PBMC)和脑脊液有核细胞(CSFMC)、53例抑郁症(DD)患者和360只绵羊PBMC中BDV p24基因片段,对阳性产物进行基因序列测定、同源性和氨基酸顺序分析;并绘制系统发生树,分析BDV感染的分子流行病学特点。结果BDV p24基因片段阳性率在VE患者CSFMC为11．54％、DD患者PBMC 11．32％明显高于健康人0％(P<0．05);绵羊的PBMC为7．78％与健康人对比差异无统计学意义(P>0．05);系统发生树图形表明,绵羊PBMC目的基因片段与人类VE和DD核苷酸序列亲缘关系最近,来源于一个分支,并与德国H1766病毒株核苷酸序列亲缘关系最近,其变异性较小。结论宁夏及其周边地区部分VE、DD可能与BDV感染有关,健康绵羊存在BDV的自然感染,其流行可能具有一定的地域局限性;人类VE、DD的BDV感染可能存在潜在动物源性,有待于进一步研究。Objective In order to investigate the epidemics of borna disease virus （BDV） in Ningxia and its vicinal regions. Methods p24 fragment of BDV from： （1） peripheral blood mononuclear cells （PBMC） and cerebrospinal fluid mononuclear cells （CSFMC） from 52 patients with viral encephalitis （VE） and 32 healthy donors, （2） peripheral blood mononuclear cells （PBMC） from 53 patients with depressive disorder （DD） and from 360 sheep, were examined by nested reverse transcriptase polymerase chain reaction（PCR） with fluorescence quantitative PCR. Gene sequence and amino acid sequence were analysed for positive product and the molecular epidemiologic characteristics by drawing phylogenetic trees. Results The positive rate of BDV p24 in CSFMC from VE （11.54%） and in PBMC from DD 11.32% was significantly higher than that in healthy donors （0 % ） （ P 〈 0.05）. The phylogenetic trees indicating the genetic relationship of the p24 fragment of BDV in both sheep and VE, DD in China and was similar to the nucleotide sequence of H1766 strain in Germany. Conclusion Data indicated that the BDV infection was possibly existing in VE, DD patients and health sheep in Ningxia and its vicinal regions with confined locality which called for further study.

关 键 词：病毒性脑炎 博尔纳病病毒 巢式逆转录酶荧光定量PCR 

分 类 号：R181.3[医药卫生—流行病学]