幽门螺杆菌中国菌株致病相关基因cagA、iceA、vacA及HP0519分布分析  

Distribution of cagA. 3' region, iceA, vacA and HP0519 on Helicobacter pylori isolated from China

作　　者：张茂俊[1] 何利华[1] 王振宇[2] 周增芬[3] 张建中[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所诊断室,北京102206 [2]香港大学皇家玛丽医院内科 [3]昆明医学院第一附属医院消化科

出　　处：《中华流行病学杂志》2006年第6期508-512,共5页Chinese Journal of Epidemiology

基　　金：国家自然科学基金资助项目(30370078)

摘　　要：目的分析中国不同人群及不同胃部疾病病例来源的幽门螺杆菌致病相关基因cagA、iceA、vacA及HP0519的分布。方法采用特异引物聚合酶链式反应(PCR)方法分析150株幽门螺杆菌上述基因的多态性分布特点,并对其分布作初步统计分析。结果93％(139／150)中国菌株cagA基因3′端重复序列的PCR产物具有东方菌株特征。75％(113／150)菌株iceA基因为iceA1, 19％(29／150)为iceA2,不同地区间iceA基因的分布差异无统计学意义。云南菌株iceA1、iceA2的分布与菌株分离个体的种族特点及临床疾病类型无显著关系。96％中国菌株(144／150)vacA基因s区的等位基因为s1;m区等位基因m2、m1b和m1b-m2的比例分别为57％(85／150)、27％(41／150)和11％(16／150),仅2株福建菌株为m1a。不同地区间vacA s1、m2、m1b分布的差异无统计学意义。云南菌株m1b-m2的分布高于福建和北京菌株。云南菌株vacA s区等位基因的多样性与分离个体的种族及临床疾病类型无显著关系。vacA m区等位基因的多样性与分离个体的临床疾病类型无显著关系,但不同民族间m2的分布有显著差异,白族人群m2的分布显著少于汉族和纳西族。93％(140／150)的中国菌株HP0519基因具有24 bp和15 bpDNA插入和缺失的多态性特点。不同地区间HP0519基因的多态性无显著不同。云南菌株HP0519的多态性与菌株分离个体的临床疾病类型无显著关系,但来源不同民族菌株的HP0519基因存在差异。结论幽门螺杆菌中国菌株cagA 3′端JF/TR特异引物的扩增结果具有东亚菌株特点。中国菌株vacA基因多为s1,其分布与菌株分离个体的临床疾病类型无关。中国菌株mcA基因m区的分布具有多样性。中国菌株HP0519基因具有24 bp和15 bp插入和缺失的多态性特点。Objective This study was aimed to characterize the Helicobacter pylori strains isolated from different geographic regions in China and different ethnic groups in Yunnan province in terms of cagA, iceA, vacA and HP0519 genes which were proposed to be related to the pathogenesis. Methods 150 Helicobacter pylori strains were collected from Yunnan province, Fujian province and Beijing. Chromosome DNA was extracted and polymerase chain reaction （PCR） was carried out to determine the 3′ region of cagA, iceA, vacA and HP0519 status with specific primers. PCR results were analyzed statistically according to their isolated original and clinical outcomes. Results For cagA 3′ region, 93 % （139/150） of the Chinese Helicobacter pylori strains belonged to East Asian type according to the specific primerof TF/JR. Among the 150 strains, 75% （113/150） belonged to iceA1, and 19% （29/150） to iceA2. The dissemination of iceA was not associated with any of the geographic regions, different ethnic groups or different clinical outcomes. 96% （144/150） of the vacA s region belonged to s1. In the vacA middle region, m2, m1b, m1b-m2 were 57% （85/150）, 27% （41/150） and 11% （16/150） respectively. However, m1a was only observed in two strains from Fujian. Neither vacA sl nor m2 showed significant difference between Yunnan, Fujian and Beijing. However, the distribution of m1b-m2 in Yunnan was higher than that in Fujian and Beijing. In Yunnan province, the distribution of vacA s1 was not associated with different ethnic groups but m2 from Bai group was less than other two ethnic groups. The ratio of m1b in Bai group was higher than that in other groups. Both vacA＇s region and m region alleles had no significant relationship with the clinical outcomes. With the 15 bp and 24 bp DNA insertion and deletion primers test, 93 % （140/150） of the strains were positive. The distributions of the 15 bp and 24 bp DNA insertion or deletion were different according to the different ethnic groups. Conclusion By JF/TR p

关 键 词：幽门螺杆菌 致病相关基因 

分 类 号：R181.3[医药卫生—流行病学]