汉坦病毒和恙虫病东方体复合感染的组织细胞培养研究  

Study on the coinfection of Hantavirus and Orientia tsutsugamushi in tissue cell culture

作　　者：邓小昭[1] 许可[2] 孔晶[1] 刁振宇[1] 钱俊英[3] 谈永飞[4] 张矛[4] 曹广文[5] 张云[1] 

机构地区：[1]南京军区联勤部军事医学研究所,210002 [2]南京医科大学 [3]陕西省疾病预防控制中心 [4]江苏省宜兴市人民医院 [5]解放军第二军医大学

出　　处：《中华流行病学杂志》2006年第6期518-521,共4页Chinese Journal of Epidemiology

基　　金：国家自然科学基金资助项目(30471491);江苏省自然科学基金资助项目(BK 2005005);全军医学"十一五"科技攻关资助项目(06G65)

摘　　要：目的了解汉坦病毒(HV)和恙虫病东方体(Ot)在宿主体内共生情况。方法采用Vero E6细胞体外培养法研究HV、Ot在宿主细胞内共生特征。结果5组感染Veto E6细胞体外培养检测结果发现:先后、同时和单纯实验感染的5组细胞中,HV和Ot先后、同时接种感染组在细胞传至第3代时检测均见有HV和Ot的双重感染,阳性率随传代次数增加而增加。而HV和Ot单纯分别感染组细胞传第2代时即可检测到HV和Ot,阳性率亦随传代次数的增加而增加。在传代培养中单纯感染组的阳性率均显著高于先后和同时感染组。其结果均用PCR和RT-PCR进行了基因鉴定。结论研究结果提示HV和Ot可在同一宿主细胞内共生,在感染初期有相互抑制作用;对HV和Ot的流行病学有一定的理论意义。Objective To investigate the possibility of Hantavirus （HV） and Orientia tsutsugamushi（Ot） coinfection in their hosts. Methods HV and Ot were used to infect Veto E6 cells cultured in vitro singly, simultaneously or successively. Genes of HV and Ot were identified in different generation cells with RT-PCR. Results Five experiment groups of infected Vero E6 cells were tested, the results were as follows： HV and Ot were both positive in infected Vero E6 cells passaged 2 times and the positive rate increased following the passaged times in HV and Ot infection groups, simultaneously or successively. However, in the groups which were infected with HV and Ot separately, the gene of HV or Ot could be detected in infected Vero E6 cells passaged only once and the positive rate increased following the times of the passaged. The positive rate was higher in the singly infected groups than in those infected simultaneously or successively. Conclusion Coinfection of HV and Ot did exist in the hosts while HV and Ot could inhibit each other in the initial infection stage.

关 键 词：汉坦病毒 恙虫病东方体 宿主 组织细胞 

分 类 号：R513.2[医药卫生—内科学] R512.8[医药卫生—临床医学]