白细胞介素1α、β及γ干扰素mRNA在子宫内膜异位症患者异位和在位子宫内膜巨噬细胞中的表达  

Expression of interleukin 1-α,β and interferon-γ in macrophages from endometrium of women with endometriosis

作　　者：尹利荣[1] 孙俊杰[1] 马洪达[2] 米淑玲[1] 郭素杰[1] 时扬[1] 

机构地区：[1]天津医科大学第二医院妇科,300211 [2]天津医科大学第二医院病理学教研室,300211

出　　处：《中华妇产科杂志》2006年第5期295-298,共4页Chinese Journal of Obstetrics and Gynecology

基　　金：天津市科学技术委员会自然科学基金资助项目(013613311)

摘　　要：目的探讨白细胞介素(IL)1α、βmRNA和γ干扰素(IFNγ)mRNA在子宫内膜异位症(内异症)患者异位和在位内膜巨噬细胞中的表达变化及意义。方法采用原位杂交技术检测40例内异症患者(内异症组)异位和在位内膜(增生期18例,分泌期22例)和15例子宫肌瘤患者(对照组)的内膜(增生期8例,分泌期7例)巨噬细胞中IL1α、β和IFNγmRNA表达水平。结果(1)内异症组患者异位、在位内膜及对照组内膜巨噬细胞中IL1αmRNA阳性表达率分别为70%(28/40)、38%(15/40)和20%(3/15),表达水平分别为3.12±0.32、2.65±0.34、1.32±0.23;IL1βmRNA阳性表达率分别为75%(30/40)、40%(16/40)和20%(3/15),表达水平分别为3.45±0.43、2.74±0.39、1.45±0.18;内异症组IL1α、βmRNA的表达水平较对照组明显升高,内异症组异位内膜较在位内膜的表达水平也明显升高,差异均有统计学意义(P<0.05)。(2)内异症组在位内膜和对照组内膜巨噬细胞中IL1α、βmRNA表达分泌期高于增生期,差异也有统计学意义(P<0.05)。(3)内异症组内膜巨噬细胞中IFNγmRNA的表达水平与对照组比较,差异无统计学意义(P>0.05),且无周期性变化。结论内异症患者在位内膜和异位内膜巨噬细胞IL1表达明显增强,而IFNγ表达则无明显变化,推测内膜巨噬细胞及其分泌的细胞因子,可能参与了内异症的发生、发展过程。Objective To assess interleukin （IL） -lα, β and interferon （IFN）γ expression in macrophages in eutopic and ectopic endometrium of women with endometriosis. Methods In situ hybridization was used to examine the expression of IL-lαand β and IFN-γin macrophages from eutopic and ectopic endometrium of 40 women with endometriosis and 15 control women. Eutopic endometrial samples were histologically classified into proliferative and secretary phases. Cervical polyp tissue was used as positive control. Re.stilts Expression of IL-lα and β in macrophages from eutopic and ectopic endometrium of women with endometriosis were significantly higher than that in the control group （ P〈0.05 ）. Both gene expression in macrophages from ectopic endometrium was higher than that in eutopic endometrium of patients with endometriosis（P〈0.05 ）. The expressions of the two genes were significantly increased in secretory phase of endometrium when compared to that in proliferative phase （ P〈0.05 ）. There was no difference in IFN-γ expression in macrophages of endometium between patients with endometriosis and control （P〉0.05 ）. No cycle dependent variation of the gene expression in the macrophages was found either in endometriosis group or in control group. Conclusion There is a significant increase in the expression of IL-lα and β in macrophages of endometriosis. IL-1 and activated macrophages may play an important role in the development and progression of endometriosis.

关 键 词：子宫内膜异位症 巨噬细胞 白细胞介素1 干扰素Ⅱ型 原位杂交 

分 类 号：R711.71[医药卫生—妇产科学]