5周递增负荷运动训练过程中T-helper1和T-helper2相关细胞因子基因表达的变化  

Changes of T-helper1/T-helper2 Special Cytokine mRNA Expression during 5 Weeks Incremental Load Training

作　　者：陶占泉[1] 陈佩杰[1] 王茹[1] 庄洁[1] 段子才[1] 

机构地区：[1]上海体育学院运动科学系,上海200438

出　　处：《中国运动医学杂志》2006年第3期271-275,共5页Chinese Journal of Sports Medicine

基　　金：国家科技攻关计划(2002BA904B04-6);上海市教委第四期重点学科建设项目;国家教育部高等学校优秀青年教师教学科研奖励计划;上海市第二期重点学科建设项目(T0901)

摘　　要：目的:从辅助性T淋巴细胞(T-helper)细胞因子mRNA表达的角度探讨递增负荷运动下机体免疫机能变化的规律。方法:采用实时定量PCR检测技术,对进行5周递增负荷训练的健康男性受试者外周血单个核细胞的T-helper特征性细胞因子mRNA进行动态观察。结果:5周递增负荷运动训练过程中,训练组实验期间IL-2mRNA变化不明显,训练2、3和4周后IL-4mRNA明显升高,训练3周、4周、5周后IFN-γmRNA、IFN-γmRNA/IL-4mRNA、PFRmRNA明显高于实验前,训练5周后受试者最大摄氧量和PWC170明显提高。对照组IL-2mRNA、IL-4mRNA、IFN-γmRNA、PFRmRNA、有氧运动能力5周实验前后均无显著性差异。结论:5周递增负荷运动训练使T-helper1/T-helper2细胞因子基因表达上调,机体免疫平衡协调能力提高。Objective To explore the exercise induced immunity changes during incremental load training from the view of the expression of helper T cells （Th） mRNA. Methods Using the technique of real - time quantified PCR to observe T - helper - special cytokine mRNA of peripheral blood mononuclear cell dynamical- ly in healthy young male who have been trained with incremental exercise for five weeks. Results There was no obvious change in IL - 2 mRNA during five weeks training. Meanwhile IL - 4 mRNA increased greatly in the second, third and fourth week. IFN - γ mRNA as well as IFN - γ mRNA/IL - 4 mRNA increased remarkably in the third, fourth and fifth week . Conclusion The expression of T - helperl/T - helper2 cytokine mRNA were up - regulated after five weeks and the subjects were gradually adapt to the increased load which indicated the enhancement of immunity function .

关 键 词：递增运动负荷训练 实时定量PCR T-helper1/T-helper2 细胞因子MRNA 

分 类 号：R87[医药卫生—运动医学]