香雪兰种子胚的组织培养和植株再生  

Tissue Culture of Embryo and Regeneration of Plant in Freesia refracta Klatt.

作　　者：王丽[1] 邹明谦[1] 王晓光 

机构地区：[1]东北师范大学遗传与细胞研究所

出　　处：《园艺学报》1996年第3期281-284,共4页Acta Horticulturae Sinica

摘　　要：香雪兰的成熟胚和未成熟胚切段，在含有ＩＡＡ２ｍｇ／Ｌ、ＮＡＡ０３ｍｇ／Ｌ和ＢＡＰ０５ｍｇ／Ｌ的改良Ｎ６培养基上可形成半透明的瘤状愈伤组织。将这种愈伤组织培养于含有ＮＡＡ０３ｍｇ／Ｌ、ＢＡＰ０５ｍｇ／Ｌ和ＫＴ０５ｍｇ／Ｌ的ＭＮ６培养基可分化出丛生芽。生根培养基为含ＮＡＡ０５ｍｇ／Ｌ的改良Ｎ６培养基。相同的外植体可在ＭＮ６＋ＩＡＡ２０ｍｇ／Ｌ＋ＢＡＰ３０ｍｇ／Ｌ培养基上通过体细胞胚胎发生途径直接形成小植株。讨论了外源激素对香雪兰种子胚离体培养的作用。Some translucent nodular calli were formed from segments of mature and immature embryos of Freesia refracta on the modified N 6 medium with 2mg/L IAA,0.3mg/L NAA and 0.5mg/L BAP. Some buds were appeared on the calli which were transferred to the MN 6 medium containing 0 3mg/L NAA, 0 5mg/L BAP and 0 5mg/L KT. The MN 6 medium with 0 5mg/L NAA was used for rooting. When segments of embryo explant were cultured on the medium containing 2mg/L IAA and 3mg/L BAP, plantlets were regenerated directly via the somatic embryogenesis. In this report, the role which exogenous hormones played in the tissue culture of embryo was also discussed.

关 键 词：香雪兰 组织培养 再生植株 

分 类 号：S682.290.3[农业科学—观赏园艺]