甜瓜抗霜霉病基因同源序列克隆与分析  

Cloning and Sequence Analysis of Resistance to Downy Mildew Gene Analogs from Melon(Cucumis melon L.)

作　　者：李金玉[1] 李冠[1] 赵惠新[2] 王贤磊[1] 颜雪[1] 

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830046 [2]新疆师范大学生命与环境科学学院,乌鲁木齐830054

出　　处：《植物生理学通讯》2006年第3期435-440,共6页Plant Physiology Communications

基　　金：新疆维吾尔自治区高校重点项目(200521102)。

摘　　要：采用RT-PCR扩增的方法,从高抗霜霉病甜瓜品种‘日本安农二号’中克隆到约3kb的cDNA片段(命名为MRGH-J),该基因是一个连续的通读编码框,编码1007个氨基酸。推测的蛋白质分子量为113.7kDa,等电点为7.88,蛋白质预测无跨膜区。根据推测的氨基酸序列,该基因属于TIR-NBS-LRR类抗病基因,具有TIR-NBS-LRR类抗病基因所有的保守结构域。核苷酸序列和氨基酸序列同源性分析结果显示,MRGH-J与甜瓜抗病基因的同源序列MRGH12及抗霜霉病相关基因mp-19均具有高达99%的同源性,推测该基因可能在甜瓜抗霜霉病中起作用。The cDNA sequence （named MRHG-j） which was 3 kb long and encoded 1 007 amino acids was cloned from the melon of high resistance to downy mildew by RT-PCR. Putative protein molecular weight was 113.7 kDa with an isoelectric point of 7.88 and no predicted transmembrane domain. It belonged to the TIR- NBS-LRR resistance gene based on putative amino acid sequence and contained all of conservative domains of TIR-NBS-LRR resistance genes. The deduced nucleic acid and amino acid sequence of MRGH-J shared 99% identity with the reported melon resistance gene analog MRGH12 and resistance to downy mildew gene mp-19, which suggested that it might be of great importance in melon disease resistance.

关 键 词：甜瓜 抗病基因 霜霉病 

分 类 号：S652[农业科学—果树学]