几种玉米基因转移技术的研究及转基因植株的获得  

Studies on Three Maize Gene Transfer Techniques

作　　者：王国英[1] 张宏[1] 丁群星 戴景瑞[1] 谢友菊[1] 

机构地区：[1]北京农业大学生物学院,北京100094

出　　处：《生物工程学报》1996年第1期45-49,共5页Chinese Journal of Biotechnology

基　　金：863项目基金

摘　　要：用基因枪、超声波和子房注射法转化玉米,所用质粒pB48.415带有3＇端截短的Bt毒蛋白基因和潮霉素磷酸转移酶(hpt)基因。用基因枪轰击玉米胚性愈伤组织和幼胚,超声波处理玉米胚性愈伤组织,用自制的微玻针注射授粉后10～20h的玉米子房,均已成功地获得了转Bt基因的玉米植株。点杂交和Southern吸印杂交的结果都证明在转基因玉米植株中存在Bt毒蛋白基因。Studies on maize transformation have been carried out through bombarding by particle gun, ultrasonicating in a DNA buffer or ovary injecting by a self-made mi-croinjector. The plasmid pB48.415, which carries a 3'-end truncated Bt-toxin protein gene and a hygromycin phosphotransferase (hpt) gene, was used in the transformation. Transgenic maize plants were obtained from immature embryos and embryogenic calli bombarded with particle gun, embryogenic calli ultrasonicated under different conditions or ovaries injected after 10-20h of pollination. The results of Dot blotting and Southern blotting analysis certified the integration of Bt gene into maize genome.

关 键 词：玉米 基因转移 转基因植株 

分 类 号：S513.032[农业科学—作物学]