白颊长臂猿染色体的研究  

A STUDY ON THE CHROMOSOMES OF WHITE-CHEEKED GIBBON(Hylobates leucogenys)

作　　者：刘瑞清[1] 佴文惠[1] 陈玉泽[1] 余定会[1] 

机构地区：[1]中国科学院昆明动物研究所细胞与分子进化开放研究实验室

出　　处：《Zoological Research》1996年第3期341-346,共6页动物学研究（英文）

摘　　要：本文对我国云南南部的白须长臂猿（Ｈ．ｌｅｕｃｏｇｅｎｙｓ）染色体的Ｇ带、Ｃ带、晚复制带及Ａｇ－ＮＯＲｓ进行了较为详细的研究。它的２ｎ＝５２，核型公式为４４（Ｍ或ＳＭ）＋６（Ａ），ＸＹ（Ｍ，Ａ）。Ｃ带表明一些染色体着丝点Ｃ带弱化；有的染色体出现插入的和端位的Ｃ带；Ｘ染色体两臂有端位Ｃ带，Ｙ染色体是Ｃ带阳性和晚复制的。Ａｇ－ＮＯＲｓ的数目，雌体有４个，雄体有５个，Ｙ染色体上具ＮＯＲ。本文对白颊长臂猿与其它长臂猿间的亲缘关系、核型进化的可能途径进行了讨论。White-cheeked gibbon(H. leucogenys) is distributed in the south of Yunnan, China.The chromosomes of a female and a male were studied by several banding techniques(G-,C -and Ag-staining), and a techniqe for simultaneous exhibiting chromosome replication patterns and late replicating Y chromosome. The diploid chromosome number is 52. There are 22 pairs of metacentrlcs or submetacentrics (No. 1-22); 3 pairs of acrocentrics(Nos. 23-25), and chromosomes Nos. 23-24 with a secondary constriction near the centromere on the short arm.The X chromosome is a large metacentric, and the Y, the smallest acrocentric. Its karyotype for mula is 44(M or SM)+ 6(A), XY(M, A). The centrimeric distribution of heterochromatin has been demonstrated in some chromosomes. In addion, there are 3 kinds of heterochromatin on the chromosomes:(1) ab sent of centromeric heterochromatin,(2) interstitial heterochromatin,(3) terminal heterochromatin.Interstitial heterochromatin has been observed on the blarm of X chromosome. Y chromosome is completely C-band positive. Silver staining revealed 5 chromosomes with NORs in the male specimen.4 Ag-NORs of theth are heated on the secondary constriction of chromosomes 23 and 24 respectivly,and one of them is located on the Y chromosome which has been confirmed by Fluorescence in situ hybridization(FISH) in the same cell. There are 4 chromosomes with Ag-NORs in the female specimen,and they are also located on the secondary constriction of chromosomes 23 and 24. Moreover,the association of Ag-NORs have been observed. Base on the above results,the relationship in gibbons and the way of chromosome evo lution in the genus Hylobates have been discussed.

关 键 词：白颊长臂猿 染色体 灵长目 

分 类 号：Q959.848[生物学—动物学]