K562细胞中锌指蛋白cDNA基因片段的克隆  

Molecular Cloning of cDNA Gene Fragments Coding for Zinc Finger Proteins Expressed in K562 Cells

作　　者：刘智[1] 朱定尔[1] 谢慎思[1] 朱小湘 肖广惠[1] 陈汉春[1] 

机构地区：[1]湖南医科大学分子生物学研究室

出　　处：《生物化学杂志》1996年第1期121-124,共4页

基　　金：国家自然科学基金

摘　　要：Ｋ５６２细胞中锌指蛋白ｃＤＮＡ基因片段的克隆刘智，朱定尔，谢慎思，朱小湘，肖广惠，陈汉春（湖南医科大学分子生物学研究室，长沙４１００７８）１９８５年，Ｍｉｌｌｅｒ等［１］等分离并测定了非洲爪蟾卵母细胞转录因子ⅢＡ（ＴＦⅢＡ）的ｃＤＮＡ序列，推出蛋白质...The total RNAs were isolated from the cultured K562 cells,and reverse transcripted with oligo (dT)_(12-18) as a primer, then amplified by PCR technique using a pair of degenerate primers characteristic of the Cys_2 His_2 type zinc finger proteins. After cloned into pUC18 vector, 13 of inserts were sequenced and searched for the sequence data in Genbank. The recombinant fragrnents of Zfg8, Zfg30, Zfg33 and Zfg51 were found to have many differences in sequences from the known genes,while others have high homology to the known gene sequences cited in the Genbank. This will serve as a basis for further cloning and studying the function of novel cDNA genes coding for zinc finger proteins expressed in leukemia K562 cells.

关 键 词：细胞 K562细胞 锌指蛋白 CDNA基因 

分 类 号：Q26[生物学—细胞生物学]