牛胚胎体外培养体系的优化  

The optimization of culture system for bovine IVF embryo

作　　者：张志平[1] 张君涛[2] 安志兴[1] 张锈[1] 张涌[1] 

机构地区：[1]西北农林科技大学生物工程研究所,陕西杨凌712100 [2]河南农业大学牧医工程学院,河南郑州450002

出　　处：《西北农林科技大学学报（自然科学版）》2006年第6期7-10,15,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"863"高技术研究发展计划项目(2001AA21380)

摘　　要：将体外受精后的牛卵母细胞随机分组进行培养,比较了胎牛血清(FBS)和发情后7 d牛血清(E 7BS)、不同培养液(CR 1+3 m g/mL BSA、SOF+3 m g/mL BSA和SOF+0.1 m g/mL PVA)、共培养体系和不同培养液体积(0.1,0.5和2.0 mL)对胚胎发育的影响,优化了牛胚胎体外培养体系,并对该体系的稳定性进行了检验。结果表明,优化后的牛胚胎培养体系为:前48 h用CR 1+3 m g/mL BSA培养液,48 h后用CR 1+体积分数10%E 7BS培养液培养120 h,培养液体积为0.5 mL,并且采用共培养,受精后颗粒细胞保留48 h。用该培养体系培养的牛胚胎发育良好,平均囊胚率为44.9%,表明该优化培养体系较稳定。This study was conducted to improve blastoeyst rate of bovine embryo in vitro. Ovaries collected from an abattoir and oocytcs were maturated and fertilizated in vitro. Then embryos were grouped randomly,the effects of different serum,culture medium （CR1 and SOF） and co culture and medium volume on embryo development were compared. The results show that the optimized- culture system for bovine embryo used 0.5 mL CR1 ＋3 mg/mL BSA for the first 48 h,then in place of the culture medium with CR1＋7th serum after bovine heat for another 120 h. Co-culture and cumuli cell holding 48 h after IVF was employed in the whole culture course. This study got mean 44.9 % blastoeyst rate and this system was stable by integrating above results.

关 键 词：牛 体外受精 胚胎培养 囊胚 血清 

分 类 号：S823.34[农业科学—畜牧学] Q813.7[农业科学—畜牧兽医]