双水相体系酶法合成L-苯丙氨酸的研究  

Preparation of L-Phenylalanine by an Aqueous TwoPhase System

作　　者：周华[1] 姚忠[1] 孙广海[1] 韦萍[1] 

机构地区：[1]南京工业大学制药与生命科学学院,江苏南京210009

出　　处：《化学反应工程与工艺》2006年第2期146-150,共5页Chemical Reaction Engineering and Technology

基　　金：国家973项目(2003CB716004);国家自然科学基金项目(20336010)

摘　　要：以天冬氨酸转氨酶为催化剂,开展了双水相体系中苯丙酮酸转氨反应制备L-苯丙氨酸的研究。双水相体系质量组成为聚乙二醇4 000(20%)/Na2HPO4(16%)。苯丙酮酸钠盐、细胞和L-苯丙氨酸在双水相体系中的分配系数分别为8.03,31.7和0.74。当底物浓度为43.4 g/L时,该体系酶法合成L-苯丙氨酸得率为78.1%,比在水溶液体系中提高了27.4%。The bioconversion of phenylpyruvate to L-phenylalanine using aspartate transaminase in an aqueous two-phase system （ATPS） was investigated. The optimal composition of an aqueous twophase system was 20% of PEG4000 with 20% of Na2HPO4. Partition coefficients of phenylpyruvate, aspartate transaminase and L-phenylalanine respectively were 8. 03, 31. 7 and 0.74 in ATPS. The enzymatic yield of L-phenylalanine in ATPS was 78.1%, increased 27. 4% higher than in aqueous system when the substrate concentration was 43.4 g/L.

关 键 词：生物转化 双水相体系 分配系数 L-苯丙氨酸 

分 类 号：O623.736[理学—有机化学] O922.2[理学—化学]