氮源对松乳菇生长及氮吸收和硝酸还原酶活性影响  

EFFECTS OF DIFFERENT NITROGEN SOURCES ON THE GROWTHNITROGEN UPTAKE AND NITRATE REDUCTASE ACTIVITIES OF ECTOMYCORRHIZAL FUNGUS LACTARIUS DELICIOUS

作　　者：廖晓初[1] 李勇[1] 袁玲[1] 黄建国[1] 

机构地区：[1]西南大学资源与环境学院,重庆400716

出　　处：《西南农业大学学报（自然科学版）》2006年第3期386-388,392,共4页Journal of Southwest Agricultural University

基　　金：国家科技部资助项目(2002BA516A17-05)

摘　　要：在供应氨态氮(NH4+-N)和硝态氮(NO3--N)的条件下,试验研究松乳菇(Lactarius delicious)3个株系(Ld-1,Ld-2,Ld-3)的生长、氮吸收和硝酸还原酶(NR)活性。结果表明,供应NO3--N和供应NH4+-N相比,前者使外生菌根的生长量显著增加,NR活性显著提高,但菌丝含氮量显著降低。此外,供应NO3--N,Ld-1和Ld-3的吸氮量显著高于供应NH4+-N;Ld-2则相反。说明松乳菇均能够吸收利用NH4+-N和NO3--N,土壤中的NO3--N适宜松乳菇生长,也可能有益于它们感染寄主植物形成外生菌根。Thee strains of Lactarius delicious, Ld-1, Ld-2 and Ld-3, were cultured on Pachlewski solid culture medium supplied with NH4^＋ -N or NO3^--N to study the effects of N sources on their growth, N uptake and nitrate reductase activity. The results showed that NO3^- -N promoted, to a greater extent, the growth and nitrate reductase activity of this fungus species as compared with NH4^＋ -N, but N content in the hyphae was decreased. Moreover, Ld-1 and Ld-3 cuhured with NO3^- -N supply showed significantly higher N uptake that with NH4^＋ -N supply, whereas the opposite was true of Ld-2, thus suggesting that the L. delicious strains can utilize both NH4^＋ -N and NO3^- -N and that NO3^- -N in soils benefits their growth and infection to host roots to form ectomycorrhizal roots.

关 键 词：松乳菇 氤 硝酸还原酶 NR活性 

分 类 号：S646[农业科学—蔬菜学]