植物冰冻切片技术的改进  

Improvement of Plant Cryo-sectioning Technique

作　　者：刘剑锋[1] 程云清[1] 阎秀峰[2] 周革[1] 王淑范[3] 

机构地区：[1]吉林师范大学生命科学学院,吉林四平136000 [2]东北林业大学生命科学学院,黑龙江哈尔滨150040 [3]四平市园林管理处,吉林四平136000

出　　处：《南京林业大学学报（自然科学版）》2006年第3期128-130,共3页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家自然科学基金资助项目(39700013)

摘　　要：以五味子叶柄、叶片、根和茎为试材进行冰冻切片,过程简单有效且所得图版清晰,在1天内即可获得高质量图片。其关键步骤是选择合适的甘油浓度、切片时的温度及展片技术。It was a time consuming process if technique of paraffin section was used to observe plant specimen because fussy processes such as dehydrating, infiltrating and embedding were needed before being observed. Cryo-sectioning technique was used to observe leafstalk, leaf, stem and root structure of Schisandra chinensis （Turez.）Ball.. The operating process was simple and efficient. Clear image could be acquired within one day. The crucial step were to select a proper concentration of glycerol, a suitable temperature of freezing and an appropriate way of section-flatting.

关 键 词：冰冻切片 改进技术 五味子 

分 类 号：S718.3[农业科学—林学] Q94-3[生物学—植物学]