γ射线对康乐霉素产生菌的诱变效应  

Mutagenesis of γ-radiation on the kanglemycin producing strain

作　　者：周建琴[1] 高荣梅[1] 

机构地区：[1]中国医学科学院中国协和医科大学医药生物技术研究所,北京100050

出　　处：《生物学杂志》2006年第3期25-27,共3页Journal of Biology

摘　　要：康乐霉素C是一个新的免疫抑制剂。为提高免疫抑制剂康乐霉素C的产量,采用γ射线对康乐霉素C产生菌———S-2-S-13的孢子进行了诱变育种。该菌株γ射线育种的条件:菌株的死亡率;变株γL i-33康乐霉素C的产量比出发菌株S-2-S-13的康乐霉素C的产量提高200%,且传至5代发酵单位仍较为稳定,说明γL i-33的稳定性较好。Kanglemycin C（K- C）is a new immunosuppressant . To improve the productivity of K- C, the spores of producing strain S - 2 - S - 13 were breeded with γ - radiation. The paper presented that the conditions of γ - radiation breeding. The experiment showed that the increase of the deadly rate of the treated spores was along with increase of radiation dosage. The K - C productivity of mutant γLi - 33 in shake bottle was increased by up to 200% than that of starting strain S-2 -S- 13 after HPLC analysis and mutant γLi -33 was also genetic stable.

关 键 词：γ射线育种 康乐霉素C 地中海诺卡氏菌康乐变株1747—64 

分 类 号：Q935[生物学—微生物学]