羧甲基葡聚糖体外抗肿瘤作用的研究  

The anti-cancer effect of carboxymethylglucan in vitro

作　　者：孙翠玲[1] 杨文鸽[1] 李花霞[2] 童丽娟[1] 潘云娣[1] 

机构地区：[1]宁波大学生命科学与生物工程学院,浙江宁波315211 [2]中国铝业股份有限公司郑州研究院,河南郑州450041

出　　处：《中国生化药物杂志》2006年第3期153-155,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：宁波市科技局青年基金资助项目(No.02J20102-19)

摘　　要：目的研究不同取代度羧甲基葡聚糖(CMG)的体外抗肿瘤作用。方法以HL-7702正常肝细胞、HepG-2肝癌细胞、MGC-803胃癌细胞为实验材料,通过四甲基偶氮唑盐(MTT)比色法分析。结果各种取代度的CMG对HL-7702的生长影响较小,但对HepG-2和MGC-803的抑制作用明显,并且随浓度升高对HepG-2、MGC-803的抑制作用也随之增强,尤以取代度为0.556的CMG2最为显著。CMG2浓度达到500μg/mL时对HepG-2的生长抑制率为54.3%,浓度为1 000μg/mL时对MGC-803抑制率达到51.3%。结论在体外实验中,取代度0.55左右的CMG能有效抑制HepG-2肝癌细胞、MGC-803胃癌细胞的生长。Purpose To detect the anti-cancer effect of carboxymethylg-lucan（CMG） with different degree of substitution（DS） in vitro. Methods Using HL-7702, HepG-2 and MGC-803 cell as the experimental objects, the anti-cancer effect of CMG was studied by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide（MTY） colorimetric Method. Results CMG weakly inhibited the growth of HL-7702, but obviously affected the growth of HepG-2 and MGC-803. The higher concentration of CMG, the bigger inhibitory effect on HepG-2 and MGC-803 cell is. The inhibitory ratio of CMG2 to HepG-2 was 54.3 % at the concentration of 500 μg/mL, to MGC-803 was 51.3% at the concentration of 1 000μg/mL. Conclusion CMG（DS≈0.55 ） could effectively inhibit the growth of HepG-2 and MGC-803 cell,

关 键 词：羧甲基葡聚糖 取代度 四甲基偶氮唑盐 抗肿瘤 

分 类 号：Q532.3[生物学—生物化学] R965[医药卫生—药理学]