罗汉果皂甙提取物对1型糖尿病小鼠细胞免疫功能的影响  

EFFECTS OF MOGROSIDE EXTRACT ON CELLULAR IMMUNE FUNCTIONS IN ALLOXAN-INDUCED DIABETIC RATS

作　　者：陈维军[1] 宋方方[2] 刘烈刚[2] 戚向阳[1] 谢笔钧[1] 宋云飞 

机构地区：[1]华中农业大学食品科技学院,武汉430070 [2]华中科技大学同济医学院营养与食品卫生学系,武汉430030 [3]桂林莱茵生物制品有限公司,桂林541300

出　　处：《营养学报》2006年第3期221-225,共5页Acta Nutrimenta Sinica

摘　　要：目的:探讨罗汉果皂甙提取物(mogrosideextract,MG)对1型糖尿病(type1diabetesmellitus,TIDM)小鼠脾脏淋巴细胞亚群及细胞因子表达的影响。方法:给予Balb/c小鼠腹腔注射200mg/kgbw四氧嘧啶造模(TIDM)。成模后按血糖随机分为,糖尿病对照组,低、高剂量MG治疗组(150、300mg/kgbw);正常小鼠也按血糖随机分为正常对照组以及低、高剂量MG组。连续灌胃30d,实验结束时测小鼠血糖水平,流式细胞仪检测脾脏淋巴细胞CD4、CD8亚群以及细胞因子IFN-γ,TNF-α和IL-4的表达,并观察胰腺组织学变化。结果:与正常对照组相比,TIDM小鼠血糖显著升高,脾脏CD8+淋巴细胞数目显著增加,CD4/CD8比值降低;IFN-γ、TNF-α的表达水平升高。MG尤其是低剂量组能降低血糖,改善胰腺的病变程度以及下调IFN-γ、TNF-α的表达水平,增加TIDM小鼠脾脏CD4+淋巴细胞数目,使CD4/CD8比值恢复正常,此外,还可显著增加正常小鼠和TIDM小鼠脾脏淋巴细胞IL-4的表达水平,但对正常小鼠其它各项指标无明显影响。结论:MG能通过免疫调节机制对TIDM小鼠脾脏淋巴细胞的抗原表达进行调控,拮抗TIDM时出现的细胞免疫功能失衡,进而对TIDM起到一定的治疗作用。Objective： To study the effect of mogroside extract on splenic lymphocytes subsets and the cytokines expression levels of type 1 diabetes mellitus （TIDM） mice. Method： Balb/c mice were rendered diabetic by a single intra-peritoneal injection of alloxan, at dose of 200 mg/kg bw. After confirmation of TIDM, control and diabetic mice were further subdivided into the following six groups, control （C）, control ＋ low dose MG （150 mg/kg bw, C-LMG）, control ＋ high dose MG （300 mg/kg bw, C-HMG）, diabetic （D）, diabetic ＋ low dose MG （150 mg/kg bw, D-LMG）, diabetic ＋high dose MG （300 mg/kg bw, D-HMG）. The MG or saline was administered by gavage daily over 30 d. At the end of the experiment, determinations of serum glucose concentration, splenic lymphocyte subpopulation and cytokines production （IFN-γ, TNF-α and IL-4） and histopathological examinations of pancreatic islets were carried out. Results： TIDM mice presented hyperglycemic manifestation and significant injury in islets. Besides, alloxan induced a marked increase in the CD8^＋ expression （thus a dramatic decrease in CD4/CD8） and production of IFN-γ, and TNF-α in splenocytes, The MG, especially at the low dose effectively attenuated the early clinical symptoms, biochemical abnormalities, pathological damages in pancreatic islets and regulated the immune dysfunction in alloxan-induced TIDM mice, possibly by influencing （up-regulating） the CD4^＋ T lymphocyte subsets and CD4/CD8 ratio, and remodeling the intracellular cytokine profiles （reducing the expression of pro-inflammatory Thl cytokines： IFN-γ, TNF-α towards a beneficial Th2 pattern）. MG therapy had no obvious effect on normal mice, except that at the low dose MG could up-regulat the IL-4 expression . Conclusion; MG could exhibit anti-diabetic effects, due to its immunity-regulating capacity on TIDM, and may be used as a new natural drug for prevention and treatment of TIDM.

关 键 词：罗汉果皂甙提取物 1型糖尿病 细胞免疫功能 

分 类 号：R285[医药卫生—中药学]