体内实验研究神经节苷脂对小鼠迟发型变态反应的影响  

The effect of ganglioside on delayed-type hypersensitivity in vivo

作　　者：孙晶[1] 李玉林[2] 李一雷[2] 

机构地区：[1]吉林大学中日联谊医院皮肤科,吉林长春130033 [2]吉林大学病理生物学教育部重点实验室

出　　处：《中国实验诊断学》2006年第6期658-660,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨神经节苷脂对小鼠迟发型变态反应的作用。方法体内利用肿瘤相关抗原皮下注射到小鼠背部皮下,每周一次,连续三周,使小鼠致敏,第四周对小鼠进行激发试验,刺激产生迟发型变态反应,即将表皮郎格罕氏细胞与神经节苷脂和肿瘤相关抗原共同孵育后注射到小鼠后足掌侧,分别于注射后24,48小时测量足掌肿胀程度来评估神经节苷脂对小鼠迟发型变态反应的作用。结果神经节苷脂可降低小鼠足掌肿胀程度,抑制小鼠迟发型变态反应。结论神经节苷脂具有抑制小鼠迟发型变态反应,同时说明神经节苷脂可以抑制表皮郎格罕氏细胞抗原提呈作用。Objective To study the effect of gangliosides on Delayed-Type Hypersensitivity in vivo.Methods In the vivo test, irranunity to the S1509a spindle cell carcinoma was induced by s.c. inoculation at weekly intervals into naive syngeneic （CAF1） for a total of three irranunizations. Delayed-type hypersensitivity was elicited 1 week after the last irranunization by injection a hind footpad with TAA-pulsed Lc incubated with or without gangliosides. Footpad swelling was assessed at 24 and 48 hours with a spring-loaded engineer＇s micrometer. Results Gangliosides suppressed Epidermal cell to elicit Delayed-type hypersensitivity against TAA in vivo test. Conclusion Ganglioside inhibit the delayed-type hypersensitivity in mice and as well as it inhibit the antigen-presenting capability of epidermal Langerhans cells.

关 键 词：神经节苷脂 迟发型变态反应 郎格罕氏细胞 

分 类 号：R392[医药卫生—免疫学]