通脉宁调控AngⅡ、LPC诱导血管平滑肌细胞c-fos、c-myc基因表达的研究  

Regulative effect of Tongmaining Capsules on expressions of cfos and c-myc genes in VSMC induced by AngII and LPC

作　　者：董建勋[1] 路广林[1] 张前[1] 牛福玲[2] 陈淑长[3] 黄启福[1] 

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学教育部重点实验室,北京100700 [3]北京中医药大学东方医院,北京100078

出　　处：《北京中医药大学学报》2006年第5期311-314,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：教育部高等学校博士学科点专项科研基金(No.200026)

摘　　要：目的观察通脉宁胶囊对血管紧张素Ⅱ(AngⅡ)、溶血磷脂酰胆碱(LPC)诱导的兔血管平滑肌细胞(VSMC)c-fos、c-myc癌基因表达的影响。方法组织贴块法培养兔主动脉平滑肌细胞,用AngⅡ、LPC诱导VSMC增殖,采用RT-PCR方法,观察通脉宁全方及益气拆方和活血拆方药物血清对AngⅡ、LPC诱导的VSMC c-fos、c-myc蛋白表达。结果AngⅡ、LPC组c-fos和c-myc蛋白表达增加,与空白组比较有显著性差异(P<0.01);通脉宁全方组、益气拆方组、活血拆方组均可使c-fos和c-myc的蛋白表达下调,与AngⅡ、LPC组比较有显著性差异(P<0.01);通脉宁全方组与益气拆方组、活血拆方组比较也有显著性差异(P<0.01)。结论通脉宁胶囊可下调AngⅡ、LPC诱导的VSMC c-fos、c-myc基因表达,而对VSMC增殖起到抑制作用。Objective To observe the influence of Tongmaining Capsules on the expressions of cancer genes, c-fos and c-myc, in rabbit vascular smooth muscle cells （VSMC） induced by angiotensin Ⅱ （AngⅡ） and lysophosphatidylcholine （LPC）. Method To culture rabbit VSMC by tissue-sticking method and than to induce the proliferation of VSMC by AngⅡ and LPC. To use RT-PCR to observe the influences of the serum of Tongmaining Capsules, its decompositions with the effect of replenishing qi or the effect of activating blood circulation, on the expressions of c-fos and c-myc genes in VSMC. Result The expressions of c-fos and c-myc genes were increased in the AngⅡ group and the LPC group with a significant difference compared with that in the blank group （ P 〈 0.01 ）. In the group of Tongmaining Capsules and groups of decompositions with the effect of replenishing qi or the effect of activating blood circulation the expressions of c-fos and c-myc genes were decreased with a significant difference compared with that of the AngⅡ group and LPC group （ P 〈0. 01 ）. There was also a significant difference among above three groups（P 〈0. 01 ）. Conclusion Tongmaining Capsules has a down-regulative effect on the expressions of c-fos and c-myc genes in VSMC induced by AnglI and LPC, therefore, it can inhibit the proliferation of VSMC.

关 键 词：通脉宁 血管平滑肌细胞 基因 血管紧张素Ⅱ 溶血磷脂酰胆碱 

分 类 号：R285.5[医药卫生—中药学]