肾络通对5/6肾切除肾衰竭大鼠残余肾组织PAI-1蛋白表达的影响  

Influence of Shenluotong on expression of PAI-1 protein in kidney remaining tissue in rats with kidney failure induced by 5/6 of nephrectomy

作　　者：尹智炜[1] 陈志强[1] 张再康[1] 丁奇峰[1] 范焕芳[1] 方敬[1] 

机构地区：[1]河北医科大学中西医结合研究所,河北050017

出　　处：《北京中医药大学学报》2006年第5期324-327,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：河北省博士基金项目(No.03547015D)

摘　　要：目的观察肾络通对5/6肾切除肾衰竭大鼠残余肾组织纤溶酶原激活物抑制物-1(PAI-1)蛋白表达的影响。方法5/6肾切除制备肾衰竭大鼠模型,随机分为假手术组、模型组、肾络通组和缬沙坦组,分别灌胃给药16周取材,应用蛋白质印迹法检测肾组织PAI-1蛋白的表达。结果模型组与假手术组相比,PAI-1蛋白的表达明显增高(P<0.01);肾络通组PAI-1蛋白表达较模型组显著降低(P<0.05),与缬沙坦组比较无明显差异。结论肾络通可下调慢性进展性纤维化肾组织PAI-1蛋白表达,改善纤溶系统的失衡,延缓5/6肾切除肾衰大鼠的病程进展。Objective To observe the influence of Shenluotong on the protein expression of plasminogen activator inhibitor 1 （PAI-1） in kidney remaining tissue in rats with kidney failure induced by 5/6 of nephrectomy, nehtod The rat model of kidney failure was established by 5/6 of nephrectomy and the sham-operation group, model group, Shenluotong group and Valsartan group were set up and treated orally respectively for 16 weeks. The expression of PAI-1 protein in kidney tissue were detected by Western blotting method in different groups. Result The expression of PAI-1 protein was increased significantly in the model group compared with that in the sham -operation group （P 〈0. 01 ）, and that in the Shenluotong group was decreased significantly （P 〈 0. 05 ） without significant difference compared with that of the Valsartan group. Conclusion Shenluotong can down-regulate the expression of PAI-1 protein in kidney tissue of chronic developing fibrosis, relieve the unbalance of fibrinolysic system, and delay the development of rat kidney failure after 5/6 of nephrectomy.

关 键 词：肾络通 肾衰竭 纤溶酶原激活物抑制物 蛋白质印迹 大鼠 

分 类 号：R285.5[医药卫生—中药学]