高效液相色谱法测定肝乐颗粒中丹酚酸B的含量  

Quantitative determination of salvianolic acid B in Ganle Capsules by HPLC

作　　者：闫永红[1] 赵婷[1] 张慧燕[2] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]国家药典委员会,北京100061

出　　处：《北京中医药大学学报》2006年第5期338-340,共3页Journal of Beijing University of Traditional Chinese Medicine

摘　　要：目的建立高效液相色谱测定肝乐颗粒中丹酚酸B含量的方法.方法 50%甲醇超声提取,YWG-C18（4.6 mm×200 mm）液相色谱柱测定,流动相为甲醇：5%乙酸水（31∶ 69）,柱温为室温,检测波长为281 nm,流速：1 mL/min.结果丹酚酸B的线性范围在80.27～401.35 μg,r=0.9998,平均回收率为97.3%,RSD为0.54（n=5）.结论利用高效液相色谱测定肝乐颗粒中丹酚酸B的含量,操作简便,结果准确,可作为该产品质量控制的方法之一.Objective To set up a HPLC method for the quantitative determination of salvianolic acid B in Ganle Granules. Method To extract salvianolic acid B with 50% methanol by ultrasound and detect with the chromatographic column of YWG - C18 （4.6 mm × 200 mm）. The mobile phase was methanol -50% acetic acid water （31：69）. The column temperature was room temperature. The detection wavelength was 281 nm and the flow rate was 1 mL/min. Result The linear range of salvianolic acid B was from 80.27 to 401.35 μg, r = 0.9998, and the average recovery rate was 97.3%. RSD was 0. 54 （n= 5）. Conclusion The procedure was easy to control with the reliable ＇result, so it can be used for controlling the quality of Ganle Granules.

关 键 词：高效液相色谱法 肝乐颗粒 丹酚酸B 

分 类 号：R284.2[医药卫生—中药学]