普伐他汀对乙酸诱导大鼠结肠炎的治疗作用及其机制的研究  

Therapeutic effects of pravastatin on colitis induced by acetic acid and relevant mechanism: Experiment with rats

作　　者：陈迟[1] 冉志华[1] 萧树东[1] 

机构地区：[1]上海交通大学医学院附属仁济医院上海市消化疾病研究所,200001

出　　处：《中华医学杂志》2006年第18期1284-1288,共5页National Medical Journal of China

基　　金：上海市重点学科建设项目基金资助(Y0205)

摘　　要：目的在乙酸诱导的大鼠结肠炎模型中,研究普伐他汀的治疗作用及其抗氧化损伤作用的机制。方法SD大鼠60只,随机分为正常对照组(10只)、模型安慰剂组(20只)、普伐他汀治疗组(15只)、柳氮磺吡啶(SASP)治疗组(15只)。正常组常规饲养,模型安慰剂组、普伐他汀治疗组、SASP治疗组8%乙酸造模后分别予以生理盐水2ml/d、普伐他汀1mg·kg-1·d-1、SASP0.25g·kg-1·d-1灌胃治疗7d,观察大鼠活动状态,进食量,体重,大便性状,大便出血情况,计算疾病活动指数(DAI),判断疗效。第8天处死大鼠并进行结肠长度测量,结肠黏膜损伤指数(CMDI)评分,组织学评级,测定组织一氧化氮(NO)自由基、脂质过氧化产物丙二醛(MDA)以及超氧化物歧化酶(SOD)含量。结果与模型安慰剂组及SASP治疗组相比,普伐他汀治疗组DAI、结肠长度、CMDI、组织学评级均有显著改善(P<0.05),NO自由基、MDA含量显著下降,SOD含量显著提高(P<0.05)。结论普伐他汀可通过抑制氧化损伤减轻结肠炎症损伤,且疗效优于传统药物SASP。Objective To investigate the therapeutic effects of pravastatin on colitis induced by acetic acid and the relevant mechanism. Methods 8% solution of acetic acid was infusion into the rectum of 50 male SD rats so as to establish models of ulcerative colitis induced by acetic acid and 10 rats were used as normal controls treated with regular feeding. Then the 50 models of ulcerative colitis were randomly assigned into 3 groups： model ＋ placebo group （ n = 20, infused intragastrically with normal saline 2 ml/d for 7 days）, model ＋ pravastatin group （ n = 15, infused intragastrically with pravastatin 1 mg·kg^-1·d^-1）, and model ＋ salicylazosulfapyridium （SASP） group （n = 15, infused intragastrically with SASP 0. 25 g ·kg^-1·d^-1）. The general conditions, food intake, weight, stool consistencies, stool bleeding of rats were observed then the disease activity index （DAI） and the therapeutic effects were evaluated. All the rats were sacrificed at day 8 with their colons taken out. The colon length, colon mucosa damage index （CMDI）, and histological score were evaluated and nitric oxide （ NO ）, malondialdehyde （ MDA ）, and superoxide dismutase （SOD） of the tissue were measured as well. Results Compared with those of the model ＋ placebo and model ＋ SASP groups, the colon length, DAI, CMDI, and histological score of the model ＋ pravastatin group were significantly improved （ all P 〈 0. 05 ）, the levels of NO and MDA were significantly reduced, while the SOD level was increased remarkably （ both P 〈 0. 05 ）. Conclusion Pravastatin may ameliorate the colitis by antioxidant activity. Its therapeutic effect is more obvious than the conventional medicine SASP.

关 键 词：普伐他汀 结肠炎 机制 抗氧化 

分 类 号：R965[医药卫生—药理学]