Y染色体非重组区HindⅢ酶切位点多态性与原发性高血压  

Polymorphic HindⅢ restriction site of the Y chromosome and essential hypertension in Chinese Han People

作　　者：李庆祥[1] 朱小玲[1] 杜如跃[1] 张宇清[2] 吴海英[2] 吴寿岭[3] 张英川[1] 郭成军[1] 颜红兵[1] 刘国仗[2] 

机构地区：[1]首都医科大学附属北京安贞医院北京心肺血管疾病研究所,北京100029 [2]中国医学科学院北京阜外心血管病医院 [3]唐山开滦矿务局医院

出　　处：《中华医学杂志》2006年第20期1390-1393,共4页National Medical Journal of China

摘　　要：目的探讨Y染色体非重组区HindⅢ酶切位点多态性与中国汉族人群原发性高血压的关系。方法入选男性研究对象857例,其中原发性高血压患者402例、正常对照人群455名。所有研究对象用常规方法提取白细胞DNA,采用多聚酶链反应结合限制性内切酶(HindⅢ)方法检测Y染色体非重组区HindⅢ酶切位点多态性。结果对照组和原发性高血压组Y染色体HindⅢ酶切位点多态性比较各基因型差异有统计学意义(P=0·02),高血压患者HindⅢ(+)者基因型为58·5%(235/402),正常人群为66·4%(302/455);高血压患者HindⅢ(+)者基因型少于正常人群,HindⅢ(-)者基因型增多。HindⅢ(+)者基因型收缩压(133·8mm Hg±25·2mm Hg),舒张压(83·5mmHg±13·3mm Hg)和平均动脉压(100·2mm Hg±16·5mm Hg)均明显低于HindⅢ(-)者基因型(分别为138·0mm Hg±27·0mm Hg、85·9mm Hg±14·4mm Hg、103·3mm Hg±17·6mm Hg,均P<0·05)。结论Y染色体HindⅢ酶切位点多态性与原发性高血压有关,可能是中国汉族人群原发性高血压的一个遗传标志。Objective To examine whether there is an association essential hypertension pressure and a polymorphic HindⅢ biallelic marker in the non-recombining region of Y chromosome in Chinese Han people. Methods Peripheral blood samples were collected from 402 males with essential hypertension pressure and 455 age- and body height-matched healthy males as control group. Genomic DNA was extracted from the white blood cells. Segments of polymorphic Hind Ⅲ restriction site of the Y chromosome were amplified from the genomic DNA by polymerase chain reaction （PCR）. The PCR products were restricted with 10 U of Hind Ⅲ overnight at 37℃. The digested products were subjected to electrophoresis in 3% agaroso gels, and stained with ethidium bromide. Results The Hind Ⅲ （ ＋ ） genotype was found in 58.5% of the men with essential hypertension（235/402） , significantly lower than that in the healthy men （64.4% , 302/455, P=0.02）. The systolic blood pressure of the men with HindⅢ（＋ ） genotype was 133.8 mm Hg± 25.2 mm Hg, significantly lower than that of the HindⅢ （ - ） genotype （138.0 mm Hg ±27.0 mm Hg, P 〈 0.05 ） , and the diastolic blood pressure （DBP） of the men with Hind Ⅲ （ ＋ ） genotype was 83.5 mm Hg ± 13.3 mm Hg, significantly lower than that of the men with Hind Ⅲ （ - ） genotype （85.9 mm Hg ±14.4 mm Hg, P = 0. 01 ） , and the mean arterial pressure of the men with Hind Ⅲ （ ＋ ） genotype was 100. 2 mm Hg ±16.5 mm Hg, significantly lower than that of the of the men with Hind Ⅲ （ ＋ ） genotype was （ 103. 3 nun Hg ± 17. 6 mm Hg, P = 0. 01 ）. Conclusion Polymorphie Hind Ⅲ restriction site of the Y chromosome is associated with essential hypertension in Chinese Han people.

关 键 词：高血压 染色体 人 多态性 限制性片段长度 

分 类 号：R544.1[医药卫生—心血管疾病] R457.7[医药卫生—内科学]