随机扩增多态性DNA分型法在铜绿假单胞菌流行病学研究方面的应用  

Randomly Amplified Polymorphic DNA in Epidemiological Study of Pseudomonas aeruginosa

作　　者：彭奕冰[1] 项明洁[1] 张健 季育华[1] 

机构地区：[1]上海第二医科大学附属瑞金医院,上海200025 [2]芜湖市二院,安徽芜湖241000

出　　处：《中华医院感染学杂志》2006年第6期605-607,共3页Chinese Journal of Nosocomiology

摘　　要：目的摸索适于铜绿假单胞菌随机扩增多态性DNA分型法(RAPD)分型的最佳实验条件,并对我院临床分离到的34株铜绿假单胞菌进行分型研究。方法从随机引物筛选、镁离子浓度、模板量、引物浓度4方面对RAPD反应体系进行优化。结果不同病区分离到的铜绿假单胞菌的指纹图谱互不相同;从烧伤病房分离到的8株高度耐药的铜绿假单胞菌中,其中7株为同一指纹图谱,另外1株为独特的指纹图谱,而外科重症监护病房、肺科病房、干部病房同一病区内分离到的铜绿假单胞菌均为同一指纹图谱。结论铜绿假单胞菌在病区内存在交叉感染的情况,但未出现病区间感染同一流行株。OBJECTIVE To establish a fingerprinting method by randomly amplified polymorphic DNA. Epidemiological study was carried out on Pseudomonas aeruginosa in Ruijin Hospital. METHODS To obtain optimum scheme on reaction system for randomly amplified polymorphic DNA （RAPD） of P. aeruginosa. RESULTS P. aeruginosa strains isolated from the same ward shared the same RAPD fingerprint type, except for pulmonary ward. Different ward was with different fingerprint type. CONCLUSIONS Prevalent strain was not found in the whole hospital, but within ward exists hospital-acquired infection phenomenon.

关 键 词：铜绿假单胞菌 随机扩增多态性DNA技术 

分 类 号：R378.991[医药卫生—病原生物学]