热力消毒灭活牙科手机污染HBV影响因素研究  

Factors Affecting Sterilization of HBV on Dental Hand Piece

作　　者：石磊[1] 牛光良 田敬华[1] 赵辉[1] 刘雅[1] 谢尧[1] 

机构地区：[1]北京地坛医院,北京100011 [2]北京市中西医结合医院,北京100039

出　　处：《中华医院感染学杂志》2006年第6期651-652,共2页Chinese Journal of Nosocomiology

基　　金：北京市科委基金资助(H010910240119)

摘　　要：目的探讨影响牙科手机污染的HBV热力学消毒因素。方法牙科手机浸泡污染HBV血清,按照不同的处理程序分组,进行牙科手机热力消毒器消毒(134℃5 min),以HBsAg检测反应表示消毒效果,样品用AXSYM自酶标、美国Abbott第三代EIA检测试剂进行HBsAg检测。结果污染血清在室温干燥后,直接热力消毒有96.87%的样品为HBsAg反应阳性,干燥后进行清洗消毒仍有87.5%不能达到灭活HBsAg的要求,即使增加抽真空的次数和延长消毒时间也有高达56.25%的样品为HBsAg检测反应阳性,而在污染后立即进行冲洗和清洗,在经过热力消毒后,均达到HBsAg检测反应阴性。结论牙科手机污染后,未进行及时清洗显著影响牙科手机热力消毒对HBsAg的灭活效果。OBJECTIVE To investigate the factors influencing the effects of disinfection of HBV on dental hand piece. METHODS After polluted with HBV positive serum, the dental hand piece was disinfected with sterilizer at the condition of 134℃ for 5 minutes according different treatment procedures. The samples were then taken and detected HBsAg by the automatic enzyme immune analyzer using the third generation EIA agent. The efficacy of disinfection of HBV was presented by the reaction of HBsAg detection. RESULTS If the dental hand piece was sterilized directly not cleansed using clean water after pollution and dried in room temperature, there were 96. 87% of the samples being HBsAg positive, and the polluted but dried hand piece, even cleansed there were 87.5% of positive result, even if prolonged the time of sterilization and the frequencies of vacuumization, the HBsAg positive rate of the sample was still up to 56. 25%. But if the hand piece was irrigated and cleansed immediately after pollution and sterilized, all samples were HBsAg negative. CONCLUSIONS Whether or not cleansed immediately is the most important factor influencing the efficaey of hand piece sterilization after pollution.

关 键 词：HBV 牙科手机 预真空灭菌器 消毒 

分 类 号：R187.5[医药卫生—流行病学]