γ-氨基丁酸B受体亚单位在人类难治性颞叶癫患者中的表达  

GABA_(B1)R expression in sections resected from patients with refractory temporal lobe epilepsy

作　　者：王乃东[1] 赵永波[1] 陈英辉[1] 王枫[1] 呙登俊[1] 

机构地区：[1]上海第一人民医院神经科,200080

出　　处：《中华神经科杂志》2006年第5期320-322,共3页Chinese Journal of Neurology

基　　金：国家自然科学基金资助项目(30370511);上海市卫生局青年基金资助项目(034Y04)

摘　　要：目的探讨γ-氨基丁酸B受体亚单位(GABAB1R)在人类难治性颞叶癫病理机制中的作用。方法采集难治性颞叶癫患者手术切除海马标本,利用逆转录聚合酶链反应(RTPCR)和蛋白免疫印迹分析(Westernblot)方法检测患者GABAB1R的mRNA和蛋白表达水平,并与非癫者进行对照。结果与对照组标本相比,难治性癫患者手术标本中GABAB1RmRNA(0.366±0.023)和蛋白(145.56±3.55)的表达均有上调(P<0.05)。结论GABAB1R可能参与了人类难治性癫的病理生理机制。Objective To explore the possible role of GABAB1 receptor, a subunit of GABAB receptor in the pathological mechanism of human refractory temporal lobe epilepsy. Methods Sections of temporal lobe were obtained from patients with refractory temporal lobe epilepsy, so were the sections of the same area from voluntarily donated bodies who were excluded of epilepsy and were age- and gender-matched with the patients. GABABI receptor and protein were measured by RT-PCR and Western-blot in the sections of both the patients and the controls. Results Both GABAB1 receptor protein （ 145.56 ±3.55 ） and mRNA （0. 366±0. 023） expression were significantly increased in patients compared with those of controls （ P 〈 0. 05 ）. Conclusion The results of this study support the hypothesis that GABAB1 receptor may participate in the pathological mechanism of human refractory temporal lobe epilepsy.

关 键 词：受体 GABA-B1 癫痫 颞叶 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R742.102[医药卫生—临床医学]