乌斯他丁对大鼠门静脉阻断后肠粘膜屏障影响的实验研究  

Effects of ulinastatin on intestinal mucosal barrier after occlusion of portal vein in rats

作　　者：徐敬[1] 何建平[2] 郑南[2] 杨福[2] 邬明[2] 沈骅[3] 

机构地区：[1]云南省第一人民医院肝胆外科,云南昆明650014 [2]昆明医学院第一附属医院肝胆外科,云南昆明650014 [3]云南省昆明市第一人民医院检验科,云南昆明650014

出　　处：《中国现代普通外科进展》2006年第3期161-164,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:探讨阻断大鼠门静脉对肠屏障功能的影响以及乌斯他丁对其损害的保护作用,为临床门静脉阻断后肠屏障功能的保护提供实验依据。方法:SD大鼠70只,随机分为对照组(n=10)、手术组(n=30)、手术+药物组(n=30)。手术组及手术+药物组门静脉阻断时间为40min,门静脉复流4h。70只动物均于手术后4h40min在无菌条件下取门静脉血2ml检测内毒素:取回盲部肠系膜淋巴结作细菌培养;距回盲部2cm处取小肠肠壁组织1cm,作病理学检查,观察肠粘膜通透性及其形态学结构改变。结果:对照组与手术组比较,大鼠门静脉阻断后其门静脉血内毒素水平明显上升;肠系膜淋巴结细菌培养阳性率增加;小肠壁形态学结构亦发生明显改变。手术+药物组与手术组比较,上述指标均有改善。结论:大鼠门静脉阻断后可导致其肠屏障功能的损害,乌斯他丁对上述损害具有保护作用。Object：TO study the effect of portal vein occlusion on Intestinal mucosal barrier In rats and the protection of ulinastatln to the Injury, to present the experimental data for the clinical surgery. Methods： 70 Sprague-Dawley rats were randomly divided Into controlled group （n=10）,operation group （n=30） and operation＋medication group （n=30）. The portal vein were occlused 40 min in the operation groups and operation＋medication groups. 2ml blood from portal vein, lymph nodes around appendix,lore small intestine wall were taken for endotoxin levels, bacterial translocation and pathlotogy examinations In the all rats 280 mins after operation.The mccusal barrier and microscopic structure of intestine were observed.Results：compared between the control group and the operation group,endotoxin leveis,bactrial transiocation rates rise greatly and gut structuse change obviously in the latter.Compared between the operation group and operation medication group,the former changes is also obvious.Conclusion：The occlusion of portal vein can leads the deerease of intestine mocusal barrier and the increase of its permeablity,Ullnaslatin has a good protective effect on the damages above.

关 键 词：门静脉阻断 肠粘膜屏障 乌斯他丁 大鼠 

分 类 号：R657.3[医药卫生—外科学] R657.5[医药卫生—临床医学]