LLN对大鼠急性心肌损伤保护作用研究  

Study on protection effect of LLN in acute myocardial injury in rats

作　　者：荆焰[1] 杨芳炬[1] 邓文龙[2] 张杰[1] 邹毅[1] 李东[1] 凌婧[1] 黄继华[1] 

机构地区：[1]四川大学基础与法医学院,成都610041 [2]四川省中药研究所,成都610041

出　　处：《中药药理与临床》2006年第1期28-30,共3页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:研究LLN对大鼠急性心肌损伤的保护作用及其部分机制。方法:用异丙肾上腺素造成大鼠心肌损伤模型。SD大鼠随机分成6组:正常组、ISO模型组(30mg/kg)、LLN大剂量组(400mg/kg)、LLN中剂量组(200mg/kg)、LLN小剂量组(100mg/kg),丹参组(400mg/kg),镜下观察药物对急性心肌损伤大鼠心肌组织病理形态学的影响;免疫组化检测对大鼠心肌细胞bcl2、bax、caspase3蛋白表达的影响。结果:ISO组心肌坏死明显,bax、caspase3蛋白表达增加;LLN组和丹参组心肌坏死减轻,bax、caspase3表达下降,bcl2/bax升高。结论:LLN对异丙肾上腺素造成的大鼠急性心肌损伤有保护作用,其机理与升高bcl2/bax比值,降低caspase3的表达和抑制凋亡有关。Objecive： To study the protection effect and possible mecbanisms of LLN in acute myocardial injury induced by isoprenaline （ISO） in rats. Methods： Acute myocardial injury was induced by hypodermic injecting ISO 30mg/kg. SD rats were divided into six groups at random. They were control group,the ISO group,the LLN group（400mg/kg） , the LLN group（200mg/kg） , the LLN group（100mg/kg） and the Danshen group （400mg/kg） ;The effect of LLN in myocardium was observed by pathomorphism. The express of bcl-2,bax,caspase-3 was studied by ABC immunohistochemistry. Results： In the ISO group, the myocardial necrosis were more serious. Moreover the express of bax, caspase- 3 increased. In the LLN group and the Danshen group, the myocardial necrosis were relieved, the The express of bax, caspase-3 decreased, bcl-2/bax increased. Conclusion： The LLN can alleviate rat myocardium from injury by increasing bcl-2/bax,decreasing the expression of caspase-3 and reducing myocardial apoptosis.

关 键 词：LLN 丹参 细胞凋亡 BCL-2 BAX CASPASE-3 

分 类 号：R285.5[医药卫生—中药学]