几个水稻粳型亲籼系成熟胚离体培养研究初报  

Preliminary Study on the Mature Embryo Culture Characteristics of Some Indica-Compatibility Japonica Lines

作　　者：傅雪琳[1] 卢永根[1] 刘向东[1] 陈竹峰[1] 

机构地区：[1]华南农业大学广东省植物分子育种重点实验室,广州510642

出　　处：《上海交通大学学报（农业科学版）》2006年第3期230-234,共5页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：广东省自然科学基金重点资助项目(021037);国家自然科学基金资助项目(30270814);华南农业大学校长基金资助项目(2001038)

摘　　要：对4个粳型亲籼系成熟胚离体培养特性研究的结果表明,种子贮存150d后的愈伤组织平均诱导率为82.02%,显著高于种子贮存20d后的诱导率(51.04%)。愈伤组织诱导率、愈伤组织质地结构、以及绿苗再生率在材料间表现出差异,其中材料间愈伤组织平均诱导率从高到低的顺序为G3004-4>G2605>G2417-2-1>G2416-3,材料之间绿苗再生率的顺序为G3004-4>G2416-3>G2605>G2417-2-1,表明供试的粳型亲籼系在S-b座位携带中性基因Sn比Si基因易于提高愈伤组织诱导率、改善愈伤组织质地结构,但S-b座位的基因型对绿苗再生率的影响不明显。不同培养基对各材料愈伤组织诱导率的影响不同,表现为在诱导培养基上添加1g·L-1水解乳蛋白和0.5g·L-1脯氨酸均能够显著提高G2417-2-1和G3004-4的诱导率;分化培养基上不同激素配比也影响其绿苗再生率。4个粳型亲籼系成熟胚离体培养特性类似于粳稻的培养特性。The mature embryo culture characteristics of four indica-compatibility japonica lines were studied.The callus induction rate of the mature embryos from seeds stored for 150 d was 82.02 %, which was obviously higher than that of the seeds stored for 20 d（51.1M %）.The callus induction rate, callus textures, and the green shoot-bud regeneration rate were different among the experimental materlals.The order of the average callus induction rate was G3004-4〉G2605〉G2417-2-1〉G2416-3, whereas the order of the average green shoot-bud regeneration rate was G3004-4〉G2416-3〉G2605〉G2417-2-1,it showed that the indica-compatibility japonica lines with gene S＂ in locus S-b were prone to raise callus induction rate and the callus textures compared to the in- dica-compatibility japonica lines with gene S^n in locus S-b.The effect of media for callus induction was different among all the materials, which presented that the callus induction rate of G2417-2-1 and G3004-4 were obviously raised by adding 1 g·L^-1 lactalbumin hydrolysate and 0.5 g·L^-1 proline into callus induction medium.The green shoot-bud regeneration rates of calli were affected by combinations of different auxins in regeneration medium.In short,the mature embryo culture characteristics of four indicacompatibility japonica lines were similar to that of the japonica cultivars.

关 键 词：粳型亲籼系 成熟胚 离体培养特性 愈伤组织 绿苗再生 

分 类 号：S511.22[农业科学—作物学]