建兰花叶病毒检测标准方法的建立  

Establish of Standard Detection Methods for Cymbidium Mosaic Virus

作　　者：杨翠云[1] 于翠[1] 郑建中[1] 陶庭典[1] 王寿南[1] 顾劲翊[1] 赵惠玲[2] 

机构地区：[1]上海出入境检验检疫局,上海200135 [2]复旦大学,上海200433

出　　处：《上海交通大学学报（农业科学版）》2006年第3期250-255,共6页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海市科委技术标准专项项目(04DZ05064)

摘　　要：经过2004￣2005两年对上海口岸进境和进境后种植的260个兰花品种上建兰花叶病毒(CyMV)检测方法的研究,分别建立了检测和鉴定兰花上建兰花叶病毒的DAS-ELISA、电镜观察、鉴别寄主反应、RT-PCR和IC-RT-PCR的方法。The studies were made for the detection methods of Cymbidium mosaic virus in 260 orchid varieties which interred Shanghai from abroad in the past two years from 2004 to 2005. The methods for detection of Cymbidium mosaic virus in orchid such as DAS-ELISA, electron microscopy observation, distinguish host reaction, RT-PCR and IC-RT-PCR were established rcspectively.

关 键 词：建兰花叶病毒 鉴别寄主反应 DAS-ELISA 电镜观察 RT-PCR IC-RT-PCR 

分 类 号：S682.31[农业科学—观赏园艺]