S-腺苷-L-蛋氨酸(SAM)稳定性盐产品制备的研究  

Study on Preparation of Stable Salts of S-Adenosyl-L-Methionine

作　　者：刘恭源[1] 崔海容 郭坚 蔡诗贵 何光源[1] 

机构地区：[1]华中科技大学中英HUST-RRes基因工程和基因组学联合实验室,武汉430074 [2]湖北出入境检验检疫局技术中心,武汉430022

出　　处：《氨基酸和生物资源》2006年第2期60-62,81,共4页Amino Acids & Biotic Resources

基　　金：国家自然科学基金(30370807);973支持项目(2002CB111302)

摘　　要：研究了S-腺苷-L-蛋氨酸(SAM)盐产品的制备方法,确定了甲醇沉淀结晶SAM硫酸盐的最优条件,进一步优化了脱色精制SAM双盐产品的方法。破胞液经超滤后,过D152树脂以硫酸溶液洗脱,可得到纯度(色谱纯)在98%以上的SAM硫酸盐产品,以对甲苯磺酸溶液溶解SAM硫酸盐后脱色精制得到SAM硫酸对甲苯磺酸双盐。Purification and preparation of stable salts of S - adenosyl - L - methionine were investigated and the optimum conditions for precipitating salts of SAM with methanol had been determined as well as the methods for preparing double salts of SAM had been improved. Being filtrated after breaking yeast cells and purified by D152 resins, eluting with H2SO4, purity of SAM （HPLC） could achieve 98%. After dissolving salts of SAM with p - toluene sulfuric acid, p - toluenesulfonate salts of SAM was obtained.

关 键 词：S-腺苷-L-蛋氨酸(SAM) 沉淀结晶 脱色 冷冻干燥 

分 类 号：Q81[生物学—生物工程]