中国南海织锦芋螺毒素的分离及鉴定  

Isolation and characterization of the conotoxins of Conus textile from the South China Sea

作　　者：肖彩[1] 包锐[2] 林原斌[1] 戴秋云[3] 

机构地区：[1]湘潭大学化学学院有机化学系,湖南湘潭411105 [2]同济大学蛋白质研究所,上海200092 [3]军事医学科学院生物工程所,北京100071

出　　处：《中国海洋药物》2006年第3期22-27,共6页Chinese Journal of Marine Drugs

摘　　要：目的从中国南海织锦芋螺中分离新的芋螺毒素,为药物发现、药物设计提供新的活性多肽或前体多肽,比较不同地域织锦芋螺毒素的构成,研究环境对织锦芋螺毒素分泌的影响。方法织锦芋螺毒液经预处理后,凝胶层析分离粗毒,用HPLC进一步分离,氨基酸测序。结果分离得到了10余个织锦芋螺毒素组分,确定了10个织锦芋螺毒素的氨基酸序列,其中4个为新型织锦芋螺毒素,6个与已报道的相同。结论中国南海织锦芋螺毒液中含有丰富的M族芋螺毒素,毒素成分与菲律宾宿务岛(Cebu)、马尼拉岛的织锦芋螺相似。Objective To isolate new conotoxins of Conus textile from the South China Sea,provide new conopeptides for drug discovery and design, and investigate the effects of living environment on toxin secretion by comparing the compositions of the conotoxins of this species from different areas. Methods Crude conotoxins were purified by Sephadex G-25 and RP-HPLC, the amino acid sequences of the purified peptides were determined. Results More than 10 conotoxins were isolated from Conus textile, 4 conotoxins were firstly isolated and 6 conotoxins had the similar sequence as the reported peptides. Conclusion Many M-family conotoxins were isolated from Conus textile, the main compositions of the venom of Conus textile from the South China Sea were similar to those from Cebu and Marinduquc island in Philippines.

关 键 词：织锦芋螺 芋螺毒素 凝胶层析 HPLC分离纯化 氨基酸序列测定 

分 类 号：R282.77[医药卫生—中药学] R284.2[医药卫生—中医学]