C4d在大鼠慢性排斥反应移植肾组织中的表达及意义  

Expression and significance of C4d in renal allograft tissue of chronic rejection in rats

作　　者：赵亚昆[1] 祝清国[1] 于泳[1] 仇宇[1] 董志[2] 高治忠[1] 

机构地区：[1]哈尔滨医科大学附属第二医院泌尿外科,黑龙江哈尔滨150086 [2]黑龙江省鸡西矿业集团杏花矿医院外科

出　　处：《中国医师杂志》2006年第6期721-723,共3页Journal of Chinese Physician

基　　金：黑龙江省哈尔滨市科技攻关计划项目(2005AA9CS1168);哈尔滨医科大学研究生创新基金

摘　　要：目的探讨大鼠肾移植慢性排斥反应模型移植肾组织中补体裂解产物C4d沉积的表达及意义。方法分别采用封闭群W istar及SD大鼠为供、受体,建立大鼠肾移植慢性排斥反应模型,随机分为试验组(非治疗组)及对照组(霉酚酸酯治疗组),观察移植术后12周各组移植肾组织学变化及C4d在肾小管周围毛细血管的沉积情况。结果术后12周,试验组出现了明显的慢性排斥反应病理改变及广泛的C4d沉积,与对照组比较病理组织学变化及C4d沉积分差异均有统计学意义(P<0.01)。结论移植肾组织中C4d的沉积可作为诊断慢性排斥反应的有效指标。Objective To investigate the expression of complement split product CAd and its significance in renal allograf tissue of chronic rejection in rats. Methods The healthy closed population Wister rats and SD rats were used as donator and acceptor in renal transplantation. The chronic rejection model of renal transplant in rats was established and the rats were divided into 2 groups. The rats in experimental group were given Mycophenolate mofetil （MMF） （10 mg/kg） and those of the control group were given nothing except CsA （5 mg· kg^-1 · d^-1 ） for 10 days. At the 12th week of renal transplantation, the allograft was tested by light microscope, and the pathological changes of renal grafts and the expression of CAd in peritubular capillaries were observed. Results On the 12th week of renal transplantation, the morphology changes of chronic rejection was observed in the experimental group and obvious CAd deposition was detected in peritubular capillaries of renal allograft tissue, with significant difference compared with those of the control group （ all P 〈 0. 01 ）. Conclusion The deposition of complement split product CAd in peritubular capillaries of renal allograft can be regarded as a significance level of chronic rejection in renal transplantation.

关 键 词：肾移植 移植物排斥 补体4 

分 类 号：R699.2[医药卫生—泌尿科学]