己烯雌酚影响雄性胎鼠睾丸引带ACTIN和PCNA表达的研究  

Influence of diethylstilbestrol on expressions of proliferating cell nuclear antigen and ACTIN in gubernaculum testis of fetal male mice

作　　者：邓汪东[1] 卢善明[1] 蒋学武[2] 

机构地区：[1]汕头大学医学院第一附属医院泌尿外科,广东汕头515041 [2]汕头大学医学院第二附属医院小儿外科

出　　处：《中国医师杂志》2006年第6期727-729,共3页Journal of Chinese Physician

基　　金：广东省自然科学基金资助项目(010438)

摘　　要：目的研究环境雌激素（EEs）的经典代表己烯雌酚（DES）对雄性胎鼠睾丸引带肌动蛋白（ACTIN）和增殖细胞核抗原（PCNA）表达的影响。方法怀孕雌性昆明小鼠60只，随机分成6组，实验组于孕9-17d分别给予DES0、25、50、100、200μg·kg^-1·d^-1，二甲基亚砜（DMSO）作为溶剂，对照组给予等体积NS。孕17d处死母鼠取出胎鼠，取下腹部，常规固定、制片，应用免疫组织化学方法检测睾丸引带ACTIN和PCNA的表达。结果实验组雄性胎鼠睾丸引带发育较差，ACTIN、PCNA表达明显降低（P〈0．05），均有显著的剂量效应关系。结论DES降低雄性胎鼠睾丸引带ACTIN和PCNA的表达，从而影响睾丸引带的收缩及增殖活性，这可能是EEs影响睾丸引带发育及导致隐睾的机制。Objective To study the effects of prenatal exposure to diethylstilbestrol （DES） on expressions of ACTIN and proliferating cell nuclear antigen （PCNA） in the development of gubernaculum testis of fetal male mice. Methods Pregnant mice were randomly divided into 6 groups and injected with DES subcutaneously from the 9th gestational day to the 17th day at dosages of 0, 25, 50, 100,200 μg·kg^-1·d^-1 dissolved in 0.2ml dimethyl sulfoxide （DMSO）. The mice in the control group were given normal saline alone. The mice were sacrificed and the fetuses were quickly removed for fixation on the 17th day. Histological changes were observed with light microscope by sliced serially in coronal plane. The expressions of ACTIN and PCNA in gubcrnaculum testis were detected by immunohistochemical methods. Results In experimental groups, the gubcrnaculum testis of mice was hypoplasia. The expressions of ACTIN and PCNA in gubcrnaculum testis were lower in the DES treated groups than those in the control group with obvious dose-effect correlation （ P 〈 0. 05 ）. Conclusion DES can inhibit the expressions of ACTIN and PCNA, which may cause the reduction of contractive activity and inhibition of cell proliferation of gubcrnaculum testis in fetal male mice.

关 键 词：己二烯雌酚 睾丸 肌动蛋白类 增殖细胞核抗原 

分 类 号：R697[医药卫生—泌尿科学]