酒精染毒后大鼠睾丸生精小管超微结构的改变  

Ultrastructural Changes of the Rat Convoluted Seminiferous Tubule after Alcohol Consumption

作　　者：金霆[1] 赵永久[1] 陈昭典[2] 沈黎明[1] 詹朝辉[1] 任福金[1] 姚吉[1] 汤忠木[1] 朱晓峰[1] 

机构地区：[1]杭州师范学院医学院附属萧山第一医院泌尿外科,浙江杭州311200 [2]浙江大学医学院附属第一医院泌尿外科,浙江杭州311000

出　　处：《中华男科学杂志》2006年第6期505-509,共5页National Journal of Andrology

基　　金：杭州市医药卫生科技项目(2002B035)

摘　　要：目的:观察酒精染毒后大鼠睾丸生精小管超微结构的变化。方法:将48只W istar雄性性成熟健康大鼠随机分为对照组(A组)、染毒组(B组),每组24只,B组用50度酒精(6 m l/kg)每天灌胃染毒1次,连续39 d,A组用生理盐水灌胃(6 m l/kg);在第14、27、40 d分别从A、B两组中各处死8只大鼠,取睾丸组织,常规电镜制片,透射电镜观察大鼠睾丸生精小管超微结构。结果:透射电镜发现:对照组各时间段睾丸生精小管超微结构无明显变化,基膜平滑、致密、厚薄均匀,支持细胞胞质丰富、粗面内织网及线粒体较多,核大、核质均匀、核仁清楚,精原细胞与基膜和支持细胞之间连接紧密,紧密连接层次清楚。染毒组第1生精周期末(相当于第14 d)超微结构开始变化;第2、3个生精周期末(分别相当于第27、40 d)超微结构变化最明显,主要表现为:①支持细胞溶酶体增多,线粒体肿胀空泡化,细胞器模糊且数量减少,支持细胞内出现较大空泡,甚至支持细胞出现萎缩;②精原细胞与支持细胞及生精小管的基膜之间出现较多较大空泡;③精原细胞凋亡,凋亡小体边集;④生精小管内的精子有过多的胞质且有大小不一的空泡,尾部断面线粒体排列不整齐、缺失或堆积;⑤紧密连接层次改变、模糊不清;⑥基膜厚薄不均,基膜外胶原组织疏松增厚,成波浪式皱褶,可见基膜断裂。结论:酒精可引起大鼠睾丸生精小管的基膜、紧密连接、支持细胞等超微结构异常,并可引起精原细胞凋亡。Objective： To study the uhrastructural changes of the rat convoluted seminiferous tubule after alcohol consumption. Methods： Forty-eight Wistar mature male rats were divided into two groups randomly： control group （A） and experimental one （B）. 6 ml/（kg·d） of 50° alcohol was perfused through the gastric tube for 39 days in Group B; and 6 ml/（kg·d） of normal saline was supplemented in Group A. The uhrastructure of the rat convoluted seminiferous tubule was observed by transmission electron micro- scope at day 14, 27 and 40. Results ： In Group A, the pykno-basement membrane was unstriated and uniform, Sertoli cells showed cytoplasmic profusion, with big nucleus, well-distributed nucleoplasm, distinct nucleolus, more mitochondria and plain hierarchical tight-junction. And the ultrastructure of the rat convoluted seminiferous tubule in Goup B began to change at the end of the first sper matlogenic cycle（D 14）and changed more and more enidently with the ethanol administration,mainly as follows：①more lysosomes and vacuolisation found in Sertoli cells ,and organelles decreasd and blurry;②more and bigger vacuoles among the spermatogonia,Sertloi cells and hasement membrane;③obvious apoptosis of spermatogonia and apoptotic bodies aggregated near the membrane;④more cytoplasm and vacuolisation in the sperm of the convoluted seminiferous tubule ,and disamanged,deleted or clustered mitochondria in the sperm tail;⑤blurry and rigid tight-junction;⑥thickened ,wrinkled or broken basement membrane and under-basement membrane.Conctusion：Alcohol can cause ultrastructural changes of the basement membrane,tight-junction and Sertoli cells of the rat convoluted seminiferous tubule and apoptosis of spermatogonia.

关 键 词：酒精 睾丸 超微结构 大鼠 雄性 

分 类 号：R114[医药卫生—卫生毒理学]