蒸馏水在精子计数和低渗肿胀试验中的应用  

Application of Distilled Water in Sperm Counting and Hypoosmotic Swelling Test

作　　者：陈芳[1] 陆金春[1] 徐会茹[1] 黄宇烽[1] 胡毓安[1] 邵永[1] 

机构地区：[1]南京大学医学院临床学院南京军区南京总医院生殖遗传实验室

出　　处：《中华男科学杂志》2006年第6期542-544,共3页National Journal of Andrology

摘　　要：目的:探讨蒸馏水在精子计数和低渗肿胀试验中的应用。方法:收集37份精液标本,每份标本分别用蒸馏水和碳酸氢钠-甲醛稀释液作1∶20稀释,然后用血细胞计数池进行计数并比较,同时记录蒸馏水稀释时精子肿胀百分率。另收集26份精液标本用于低渗肿胀试验结果的比较。结果:蒸馏水作为计数稀释液与碳酸氢钠-甲醛液相比,精子计数结果差异无显著性(P>0.05)。蒸馏水稀释时所得的精子肿胀百分率与WHO推荐的低渗肿胀试验结果亦无差异(P>0.05)。结论:蒸馏水不仅可取代碳酸氢钠-甲醛稀释液作为精子计数稀释液,亦可取代低渗肿胀液用于低渗肿胀试验分析。Objective： To evaluate the application of distilled water in sperm-counting and hypoosmotic swelling test. Methods： Thirty-seven semen samples were collected and each was diluted by distilled water and sodium acid carbonate-formaldehyde solution, respectively. Then the hemacytometer was used for sperm counting. Meanwhile, the percentage of swelled sperm diluted by distilled water was compared with the result of hypoosmotic swelling test recommended by WHO. Another 26 semen samples were diluted by distilled water and hypoosmotic swelling solution respectively, and the percentages of the swelled sperm were compared. Results ： There was no significant difference either between the sperm concentrations obtained by distilled water and sodium acid carbonate-formaldehyde solution （P〉0.05 ） or between the percentages of the swelled sperm diluted by distilled water and hypoosmotic swelling solution. Conclusion： Distilled water can not only replace sodium acid carbonate-formaldehyde solution for sperm-counting dilution but also be used as a hypoosmotic swelling solution.

关 键 词：蒸馏水 精子计数 低渗肿胀试验 

分 类 号：R446.19[医药卫生—诊断学]