阿托品对四氧嘧啶糖尿病动物模型的影响  

Effect of alloxan combined with atropine on induction of diabetes mellitus in rodents

作　　者：梁志锋[1] 林军[2] 黄晶[3] 杨斌[2] 韦康来[4] 柯美珍[1] 陈美芳[2] 

机构地区：[1]广西医科大学基础医学院生理教研室,广西壮族自治区南宁市530021 [2]广西医科大学药学院药理教研室,广西壮族自治区南宁市530021 [3]广西医科大学学工处02级14班,广西壮族自治区南宁市530021 [4]广西医科大学基础医学院病理教研室,广西壮族自治区南宁市530021

出　　处：《中国临床康复》2006年第28期76-78,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:探讨四氧嘧啶配伍阿托品对制作糖尿病动物模型的影响。方法:实验于2005-07/09在广西医科大学药学院药理实验室完成。选取小鼠40只禁食12h,随机分为对照组、四氧嘧啶组(50mg/kg腹腔注射×2)、四氧嘧啶配伍高剂量(2mg/kg腹腔注射×4)阿托品组、四氧嘧啶配伍中剂量(1mg/kg腹腔注射×4)阿托品组,各10只。配伍阿托品组分别于注射四氧嘧啶前0.5h,后0.5h,2,3d共4次给予腹腔注射阿托品,全部在造模后4,14d测定空腹血糖。选取大鼠58只禁食20h,随机分为高剂量(130mg/kg腹腔注射×2)四氧嘧啶组、中剂量(100mg/kg腹腔注射×2)四氧嘧啶组、高剂量四氧嘧啶配伍阿托品组、中剂量四氧嘧啶配伍阿托品组和对照组,各组分别为14,10,14,10,10只。除对照组注射等量生理盐水外,其余各组均注射四氧嘧啶,分别于注射四氧嘧啶前0.5h,后0.5h,2,3d共4次给予腹腔注射阿托品(2mg/kg),并于造模后4,14,30d测定空腹血糖,以空腹血糖≥11.1mmol/L判定为糖尿病模型。测定各组动物的空腹血糖及血糖缓解幅度[血糖缓解幅度(%)=(前高血糖数值-后高血糖数值)/前高血糖数值×100%],计算糖尿病大鼠成模率、累计死亡率、平均存活时间。结果:纳入小鼠40只和大鼠58只,38只小鼠和51只大鼠进入结果分析。其中造模第2天四氧嘧啶组小鼠死亡1只,采血时四氧嘧啶配伍中剂量阿托品组小鼠死亡1只;采血测血糖前高剂量四氧嘧啶组和高剂量四氧嘧啶配伍阿托品组大鼠分别累计死亡2只和3只,采血时此两组又各死亡1只。①小鼠四氧嘧啶配伍高剂量和中剂量阿托品组高血糖缓解幅度均较单用四氧嘧啶组明显(分别为10.3%,10.6%,47.5%,P<0.01),高剂量阿托品血糖稳定效果更佳。②四氧嘧啶配伍高剂量阿托品,造模后4d大鼠血糖水平升幅较四氧嘧啶组小[四氧嘧啶配伍高剂量阿托品组、四氧嘧啶组分别为(11.6±3.6),(21.0±6.7)mmol/L],造模后30d血AIM： To explore the effect of alloxan combined with atropine on model establishment of diabetes mellitus in rodents. METHODS： The experiment was carried out in the pharmacological laboratory of Pharmacy College, Guangxi Medical University from July to September 2005. Totally 40 mice were randomly divided into control group, alloxan group （intraperitoneal injection with alloxan 50 mg/kg for 2 times）, alloxan combined with high dose atropine group （intraperitoneal injection with atropine 2 mg/kg for 4 times） and alloxan combined with middle dose atropine group （intraperitoneal injection with atropine 1 mg/kg for 4 times） after 12 hours of fasting. The mice were intrapertoneally injected with atropine at hour 0.5 before, hour 0.5 after, days 2 and 3 after injection of alloxan, respectively, to establish the alloxan combined with atropine groups. The fasting blood-glucose （FBG） was measured on the 4^th and 14^th days after injection of alloxan. Meanwhile, a total of 58 rats were randomly divided into high dose alloxan group （intraperitoneal injection 130 mg/kg for 2 days, n=14）, middle dose ailoxan group （intraperitoneal injection 100 mg/kg for 2 days, n=10）, high dose alloxan combined with atropine group （n=14）, middle dose ailoxan combined with atropine group （n=10） and control group （n=10）. The rats except for the control group （intraperitoneal injection with normal saline） were intraperitoneally injected with atropine （2 mg/kg at hour 0.5 before, hour 0.5 after, days 2 and 3 after injection of alloxan, respectively, to measure the FBG on the 4^th, 14^th and 30^th days after alloxan injection, and the rats were considered as diabetes mellitus （DM） when FBG ≥11.1 mmol/l., The FBG and hyperglycemia remission amplitude （HRA） were measured [HRA （%）=（former hyperglycemia-later hyperglycemia）/former hyperglycemia× 100%], and the established rate of DM rat model cumulative death rate and mean survival time were also calculated. RESULTS： Among the 40 m

关 键 词：糖尿病 四氧嘧啶 阿托品 小鼠 大鼠 疾病模型 动物 

分 类 号：R587.1[医药卫生—内分泌]