当归芍药散对快速老化小鼠脑内淀粉样颗粒沉积的影响  

Effects of Danggui Shaoyao San on the amyloid granules of senescence accelerated mouse brain

作　　者：李泽良[1] 何宏文[2] 曲怀刚[2] 张圆圆[2] 袁群芳[2] 

机构地区：[1]顺德职业技术学院医学系,顺德528300 [2]中山大学基础医学院解剖教研室,广州510080

出　　处：《解剖学研究》2006年第2期90-93,共4页Anatomy Research

基　　金：国家自然科学基金(No.30472248);广东省中医药管理局基金(No.103061)资助项目

摘　　要：目的观察当归芍药散对快速老化小鼠脑内淀粉样颗粒沉积的影响,为当归芍药散治疗阿尔采默病提供实验依据。方法选用SAM-P/8小鼠16只(6月龄),随机分为治疗组和对照组,治疗组灌服当归芍药散水煎液1个月。用六胺银法显示淀粉样颗粒,对银染颗粒进行图像分析。结果银染颗粒主要成群分布于海马;治疗组和预防组的颗粒直径,面积和灰度均较对照组明显减小。结论当归芍药散可减少快速老化小鼠脑内淀粉样颗粒的大小和密度,显示当归芍药散对脑内淀粉样颗粒的沉积可能有抑制作用。Objective To observe the effect of Danggni Shaoyao San （DSS） on the amyloid granules in senescence accelerated mouse brain, and provide scientific basis for the treatment of Alzheimer＇s disease by it. Methods Sixteen SAM-P8 mice were randomly divided into treatment group and control group. The treatment group were administered with decoction of DSS for 1 month. Then the mouse brains were stained by Gomori＇s methenamine sliver nitrate. The density （grey scale）, diameter and area of the methenamine sliver strain granules （MSSG） of hippocampus were analyzed by photographs auto-analyzing system. Results MSSG clustered predominantly in the hippocampus. The mean diameter and density of MSSG and the mean number of the dusters were significantly decreased in treatment group and prevention group. Conclusion Danggni Shaoyao San can diminish the clusters of MSSG in the hippocampus, it suggests that the deposition of the amyloid granules in senescence accelerated mouse brain is probably inhibited by Danggni Shaoyao San.

关 键 词：阿尔茨海默病 当归芍药散 Β-淀粉样蛋白 快速老化小鼠 六胺银法染色颗粒 

分 类 号：R285.5[医药卫生—中药学]