小鼠心脏神经嵴细胞的体外培养及其生物学特性  

In vitro Culture and Biological Properties of Cardiac Neural Crest Cells of Mouse Embryo

作　　者：彭涛[1] 黄国英[1] 赵晓晴[1] 谢利剑[1] 齐春华[1] 周国民[2] 

机构地区：[1]复旦大学儿科医院心血管中心,上海200032 [2]复旦大学解剖与组织胚胎学系,上海200032

出　　处：《中国实验动物学报》2006年第2期81-83,I0001,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(编号:30471823)

摘　　要：目的体外培养和鉴定心脏神经嵴细胞,探讨其生物学特性。方法取8·5d小鼠胚胎枕中部至第3体节神经管,组织块法无血清条件培养获心脏神经嵴细胞,采用转录激活因子2α(AP-2α)作为其生物学标记物,观察其迁移、分化等生物学特性。结果从胎鼠神经管中分离培养的细胞AP-2α表达阳性,具有迁移特性,传代后以含血清培养基培养后能自然分化成神经元和神经胶质细胞。结论体外培养可成功获得心脏神经嵴细胞,且具有迁移特性和多向潜能分化能力。Objective To establish an in vitro culture method of cardiac neural crest cells of mouse embryo and characterize their biological properties. Methods Neural tube isolated from the region between midotic placode and third somite of mouse embryo at E 8.5 was cultured in DMEM/F12. AP-2α was used as a biological marker of cardiac neural crest cells and detected by immunocytochemistry. The characteristics of migration and multi-directional differentiation were observed. Results AP-2α was expressed abundantly in the cultured neural crest cells, which were able to migrate and to differentiate into neurons and astrocytes as well. Conclusion The cardiac neural crest cells of mouse embryo can be successfully cultured in vitro. These cells have ability of migration and potential of multi-directional differentiation.

关 键 词：心脏 神经嵴 生物学 细胞分化 

分 类 号：R541.1[医药卫生—心血管疾病]