EFFECTS OF ARSENIC TRIOXIDE ADMINISTRATION STYLES ON LEUKOCYTOSIS  

作　　者：Jin Zhou Ran Meng Xin-hua Sui Lu Meng Bao-feng Yang 

机构地区：[1]Department of Internal Medicine, the First Hospital of Harbin Medical University, Harbin 150001 [2]Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing 100053 [3]Department of Surgery, Mudanjiang Medical College, Mudanjiang 157011 [4]Department of Biochemistry ＆ Molecular Biology, University of Georgia, GA 30602, USA

出　　处：《Chinese Medical Sciences Journal》2006年第2期111-114,共4页中国医学科学杂志（英文版）

基　　金：Supported by National Natural Science Foundation (30370507) ;Heilongjiang Excellent Youth Foundation (2003AA9CS188-12).

摘　　要：Objective To study the effects of constantly slow intravenous arsenic trioxide （As2O2） infusion regimen on decreasing leukocytosis in vivo and in vitro. Methods Three kinds of leukemia cells, NB4, K562, and acute promyelocyfic leukemia （APL） cells, were cultured in the media with constant concentration and varying concentrations of As2O3 respectively for 24 hours. Seventyfive patients were enrolled in two groups randomly. In trial group, 37 patients received continuously slow intravenous As2O3 infusion regimen for 24 hours with an infusion rate of 8 drips per minute and total infusion duration of about 18- 21 hours daily. In control group, 38 patients received routine regimen with an infusion rate of 45-55 drips per minute and total infusion duration of about 2-3 hours daily for 24 hours. The daily As2O3 dosage was 0. 16 mg/kg. The intracellular arsenic concentration was measured by atomic fluorescence assay. The apoptosis rate of cells, CD33 ^- CD11b ^＋ cells, and CD33^ ＋ CD11b^ - cells were monitored by flow cytometry. Results The apoptosis rates of NB4, K562, and APL leukemia cells in the media with constant As2O3 concentration were 56.6% ±2.4%, 27.6% ±3.1%, and 52.2% ±2. 8%, respectively, which were significantly higher than those with changing As2O3 concentration （23.2% ±2. 1%, 11.0% ±2.5%, and 21.0% ±2.5%, respectively, P〈 0.01）. The apoptosis rates of APL, M2 type acute myeloid leukemia （AML-M2）, and chronic myeloid leukemia （CML） patients in the trial group （28.5% ±1.9%, 9.5% ±0.6%, and 12.5% ±1.8% ） were also significantly higher than those in control group （8.5% ± 2. 2%, 2. 9% ± 0. 8%, and 4. 5% ± 1.2% ; P 〈 0.05 ）. The ratios of CD33 ~ CD11b^- and CD33^- CD11b^＋ cells in control group were significantly higher than those in trial group. Conclusion The continuously slow intravenous As2O3 infusion regimen can obtain high efficiency of apoptosis and low differentiation proportion, relieve leukocytosis, and gain maximal therapeutic benefit.

关 键 词：arsenical infusion regimen LEUKOCYTOSIS 

分 类 号：R557[医药卫生—血液循环系统疾病]