配液结晶法制备溶菌酶蛋白质晶体的生长机理研究  

Study on Growth Mechanism of Lysozyme Crystal Grown by Batch Crystallization Method

作　　者：于泳[1] 陈万春[1] 康琦[1] 刘道丹[1] 戴国亮[1] 崔海亮[1] 

机构地区：[1]中国科学院力学研究所微重力实验室,北京100080

出　　处：《化学学报》2006年第12期1284-1290,共7页Acta Chimica Sinica

基　　金：中国科学院知识创新工程(Nos.KSCX2-SW-322;KJCX2-SW-L05);国家自然科学基金(No.10472127);国家自然科学基金重点项目(No.10432060)资助项目.

摘　　要：采用配液结晶法制取了溶菌酶蛋白质晶体,使用动态光散射测量了溶液中聚集体的颗粒度几率分布;使用Zeiss显微镜测定了溶菌酶(110)晶面的生长速度.实验表明:随着蛋白质和NaCl浓度的增加,溶液中聚集体的颗粒尺寸也相应增加.随着反应时间的增加,溶菌酶分子在溶液中的聚集反应,逐渐达到平衡;在蛋白质和NaCl浓度较高时,溶菌酶晶体的(110)面生长较快,而在蛋白质和NaCl浓度较低时,该晶面生长较慢.基于二维成核生长机理,从晶体生长动力学理论方程出发,计算了二维成核的形成能α=4.01×10-8J?cm-2.The lysozyme crystals were grown by batch crystallization method. The distribution of the aggregates particles in the lysozyme solution was measured by dynamic light scattering （DLS）. The growth rate of lysozyme crystal was obtained by Zeiss microscope. The experimental results showed that the dimension of aggregate particles was increased with the concentration of protein and NaCI in the solution. The aggregation reactions of lysozyme molecules in the solution were increased with time gradually and arrived at equilibrium finally. The finding indicated that the higher the concentration of protein and NaCl, the faster the growth rate of （110） face. According to kinetics of crystal growth, the formation energy of two-dimensional nucleation, a was calculated to be about 4.01 × 10^-8 J·cm^-2 based on two-dimensional nucleation mechanism.

关 键 词：蛋白质晶体 晶体生长机制 动态光散射 

分 类 号：Q814[生物学—生物工程]