黄瓜花叶病毒2个分离物的亚组鉴定及株系分化研究  

Subgrouping identification and strain differentiation of two Cucumber mosaic virus isolates

作　　者：席德慧[1] 林宏辉[1] 向本春[2] 

机构地区：[1]生物资源与生态环境教育部重点实验室四川大学生命科学学院,成都610064 [2]石河子大学农学院植物保护系,石河子832003

出　　处：《植物病理学报》2006年第3期232-237,共6页Acta Phytopathologica Sinica

基　　金：教育部博士点基金(20040610015);教育部优秀人才支持计划;四川省科技厅基金(04ZQ026-036);成都市科技攻关项目(05HJSW117)

摘　　要：基于黄瓜花叶病毒甜菜分离物CMV-X J1和CMV-X J2独特的生物学特性,利用RT-PCR对接种寄主植物进行了检测。RT-PCR产物的RFLP结果显示,2个病毒分离物的M spI酶切图谱与CMV亚组I病毒的酶切图谱很相似,但经EcoR I酶切后出现显著差异;进一步对2个分离物的外壳蛋白基因进行了克隆,序列分析表明,CMV-X J1和CMV-X J2归属CMVIB亚组,二者存在着株系分化的趋势。Based on particular biological characteristics of Cucumber mosaic virus beet isolates CMV-XJ1 and CMV-XJ2, detection was done by RT-PCR. RFLP of RT-PCR products showed that, the resulting fragments digested with Msp Ⅰ of the two isolates were very similar to isolate belonging to subgroup Ⅰ, but distinct difference appeared when they digested with EcoR Ⅰ. Thus CP gene of CMV-XJ1 and CMV-XJ2 were cloned. Sequence analysis revealed both were belonging to CMV subgroup IB, there existed differentiation trend between them.

关 键 词：黄瓜花叶病毒 RT-PCR-RFLP 序列分析 亚组鉴定 株系分化 

分 类 号：S432.41[农业科学—植物病理学]