知母提取物对马铃薯晚疫病菌的抑制作用及防病效果  

Inhibition of Anemarrhena asphodeloides Bunge extract(AAE) against Phytophthora infestans and control efficacy on potato late blight

作　　者：王树桐[1] 曹克强[1] 胡同乐[1] 张凤巧[1] 

机构地区：[1]河北农业大学植物保护学院河北省病虫害生物防治工程技术研究中心,保定071001

出　　处：《植物病理学报》2006年第3期267-272,共6页Acta Phytopathologica Sinica

基　　金：河北省科技攻关项目(01220173D);河北农业大学博士基金项目

摘　　要：本研究测试了知母提取物对马铃薯晚疫病菌菌丝生长、游动孢子释放、休止孢萌发和孢子囊直接萌发的抑制作用。结果表明,知母的水提取物对菌丝生长、游动孢子释放和休止孢萌发的抑制中浓度分别为1.91 mg/mL、1.23 mg/mL和1.04 mg/mL;对孢子囊直接萌发也具一定的抑制作用,在10.00 mg/mL浓度下,抑制率达72.89%。离体叶片测试发现,浓度高于0.5%的知母提取物对马铃薯晚疫病具有显著的防治效果。盆栽植株测试结果表明,1%的知母提取物对马铃薯晚疫病的保护效果达70%以上,防效优于离体叶片试验结果,且持效期可达5 d。2%和1%的知母提取物对马铃薯晚疫病的治疗效果不明显。Anemarrhena asphodeloides Bunge is a kind of traditional Chinese medicinal herb. Inhibition of A. asphodeloides Bunge extract （AAE） against Phytophthora infestans were tested in vitro. The results showed that the effective concentration （EC50） values of AAE against mycelium growth, zoospore release and cystospore germination were 1.91 mg/mL, 1.23 mg/mL and 1.04 mg/mL respectively. The inhibitory effect of AAE against sporangia germination was 72.89% at the concentration 10.00 mg/mL. Control effects on potato late blight （PLB） of AAE were tested on detached-leaf trials and potted plant trials. The results showed that significant control efficacy against PLB were found when concentration of AAE was over 0.5%. Preventative control efficacy could be over 70% when the concentration of AAE at 1%, and the active duration time was 5 days. However, no significant curative control effect was found in the tests at the concentration 1% and 2%.

关 键 词：知母提取物 马铃薯晚疫病菌 抑菌作用 病害防治 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]