成人骨髓间充质干细胞体外分化为胰岛β细胞的初步研究  

The Preliminary Study on Induction of the Adult Bone Marrow Mesenchymal Stem Cells to Differentiate into Beta-Cells in Vitro

作　　者：王志伟[1] 钱海鑫[1] 王勇[2] 陆玉华[2] 

机构地区：[1]苏州大学附属第一医院普外科,江苏苏州215006 [2]南通大学附属医院普外科,江苏南通226001

出　　处：《苏州大学学报（医学版）》2006年第3期378-381,共4页Suzhou University Journal of Medical Science

摘　　要：目的研究成人骨髓间充质干细胞体外诱导分化成为胰岛β细胞的可能性。方法采用Percoll分离液和差异贴壁法分离纯化成人骨髓间充质干细胞,在纤维连接蛋白包被,含有表皮生长因子和血小板源性生长因子BB的低浓度血清(2%FBS)培养液中培养,传代后用碱性成纤维细胞生长因子、B27添加剂、尼克酰胺诱导。放免法检测培养液中胰岛素,双硫腙和免疫细胞化学进行鉴定,并进行体外细胞功能测定。结果细胞诱导第二周逐渐聚集成胰岛样细胞团,开始分泌胰岛素,双硫腙染色成猩红色,免疫荧光显示细胞表达胰岛素,体外葡萄糖刺激胰岛素释放试验阳性。结论成人骨髓间充质干细胞可以在体外一定条件下实现向β细胞转分化。Objective To study the feasibility of differentiation of adult bone marrow mesenchymal stem cells （MSCs） into β cells in vitro. Methods MSCs were isolated from adult bone marrow by Percoll, purified by their adhesiveness, cultured on fibroneetin with epidermal growth factor and platelet-derived growth factor BB and 2 % fetal bovine serum, the descended cells were induced by basic fibroblast growth factor, B27 supplement, nicotinamide. Insulin in the medium was detected by RIA, the cells were identified by dithizone（DTZ）and immunocytochemistry, and the cell function was detected in vitro. Results Two weeks after induction, MSCs began to gather as clusters similar to pancreatic islets, and secrete insulin, and could he stained with DTZ; the expression of insulin was visualized by immunofluorescence, and the clusters were positive for the test of glucose-stimulated insulin secretion in vitro. Conclusion The adult bone marrow MSCs can be induced to differentiate into β cells under a certain cultured condition in vitro.

关 键 词：间充质干细胞 骨髓 Β细胞 分化 

分 类 号：R338.1[医药卫生—人体生理学] R651.2[医药卫生—基础医学]