葡萄糖神经酰胺合成酶在人白血病细胞中的表达及其与多药耐药的关系  

Expression of Glucosylceramide Synthase mRNA in Human Leukemia Cell and Its Relation to Multidrug Resistance

作　　者：葛素梅[1] 谢平[2] 谢可鸣[1] 沈云峰[2] 顾中华[2] 穆会君[2] 王珏[2] 张滨[2] 

机构地区：[1]苏州大学医学院病理生理学教研室,江苏苏州215007 [2]无锡市第一人民医院中心实验室,江苏无锡214002

出　　处：《苏州大学学报（医学版）》2006年第3期386-388,395,共4页Suzhou University Journal of Medical Science

摘　　要：目的观察葡萄糖神经酰胺合成酶(glucosylceramide synthase,GCS)基因在人白血病细胞的表达及其与肿瘤多药耐药的关系。方法采用RT-PCR方法分析耐药/非耐药白血病患者GCS基因和多药耐药(multidrug re-sistance,MDR)基因(mdr1)的表达水平及其两者的差异。结果白血病耐药组GCS基因扩增条带光密度相对比值明显高于非耐药组(P<0.05);白血病患者标本GCS基因扩增条带显著增强时往往伴有mdr1基因的表达,两者呈正相关(P<0.01、γ=0.6)。结论白血病多药耐药患者GCS基因的表达明显强于非耐药患者,GCS基因的表达与mdr1基因相关。提示GCS基因可能在白血病多药耐药中起着重要作用。Objective To investigate the difference of glucosylceramide synthase （GCS） mRNA expression between drug-sensitive and drug-resistant human leukemia and to study the relationship between glucosylceramide synthase （GCS） mRNA and multidrug resistance gene （mdr1） in drug-resistant human leukemia. Methods Reverse transcriptional polymerase chain reaction （RT-PCR） was employed to analyze the expression of GCS and mdr1 mRNA in drug-sensitive and drug-resistant human leukemia. Results The comparative optical density ratio of the amplified straps in the drug- resistant group were significantly higher than one in the drug-sensitive group （ P 〈 0.05）. There was positive correlation between the expression of GCS gene and mdr1 gene in leukemia samples （P 〈 0.01, y = 0.6）. Conclusion GCS gene may play an important role in MDR during tumor progression.

关 键 词：白血病 多药耐药 葡萄糖神经酰胺合成酶基因 逆转录聚合酶链反应 

分 类 号：R557[医药卫生—血液循环系统疾病]